Journal of Advanced Biomedical Sciences

en ارتباط پلی مورفیسم های ژن آدیپونکتین rs17300539، rs266729 و rs1501299 با سطوح آدیپونکتین، مقاومت به انسولین و بیماری کبد چرب غیر الکلی در یک جمعیت ایرانی Association of Adiponectin Gene Polymorphisms rs17300539, rs266729 and rs1501299 with Adiponectin Levels, Insulin Resistance and Non-Alcoholic Fatty Liver Disease, in an Iranian Population بيوشيمي بالینی Biochemistry پژوهشي Research <div style="text-align: justify;">Background & Objectives: In this study, we investigated the relationship between polymorphisms in the adiponectin gene and biochemical parameters—specifically adiponectin levels, insulin resistance, and the presence of non-alcoholic fatty liver disease (NAFLD)—in an Iranian population. Materials & Methods: We conducted a case–control study comprising 80 individuals with NAFLD and 80 healthy controls. Genotyping of the ADIPOQ gene polymorphisms rs17300539, rs266729, and rs1501299 was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method, while serum adiponectin and insulin levels were quantified via enzyme linked immunosorbent assay (ELISA). Results: Our findings demonstrated that patients exhibited significantly higher serum triglyceride levels, fasting blood glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and diastolic blood pressure compared to healthy individuals. In contrast, adiponectin and high density lipoprotein cholesterol (HDL-C) levels were significantly lower in patients, and body mass index (BMI) was significantly elevated (P < 0.05). None of the analyzed single nucleotide polymorphisms (SNPs) were associated with insulin resistance. However, there was a significant difference in both genotype and allele frequencies of the rs1501299 variant between the patient and control groups. Additionally, within the case group for rs17300539, GA carriers had a higher BMI than GG carriers. Moreover, significant associations were observed between the rs17300539 and rs266729 polymorphisms and AST levels (P < 0.05). Conclusions: Our results suggest that the G allele of rs17300539 in the adiponectin gene may play a protective role by reducing the complications associated with NAFLD, while the rs1501299 polymorphism appears to be associated with an increased risk of NAFLD.  </div> Non-alcoholic fatty liver, Adiponectin, polymorphism, Fatty tissue, Insulin resistance. 102 115 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2379-3&slc_lang=en&sid=1 Fahimeh Rezaie فهیمه رضایی fahimehrezaei1392@gmail.com 100319475328460029518 100319475328460029518 No Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran گروه زیست شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران Masoumeh Nezhadali معصومه نژادعلی ma_nejadali@yahoo.com 100319475328460029519 100319475328460029519 Yes Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran گروه زیست شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران Mehdi Hedayati مهدی هدایتی hedayati47@gmail.com 100319475328460029520 100319475328460029520 No Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Science, Shahid Beheshti University of Medical Science, Tehran, Iran مرکز تحقیقات غدد سلولی و مولکولی، پژوهشکده علوم غدد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران Leila AmirMohammadi لیلا امیرمحمدی ieilaamirmohammadi@gmail.com 100319475328460029521 0009-0009-3778-2003 No Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran گروه زیست شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران Javad Rastgar moghadam جواد رستگار مقدم rg.ehtesham@yahoo.com 100319475328460029522 0009-0008-5240-2243 No Faculty of Medicine, Medical Tehran Branch, Islamic Azad University, Tehran, Iran دانشکده پزشکی، واحد تهران، دانشگاه آزاد اسلامی، تهران، ایران