en بررسی همبستگی بین بیان ژن clumping factor A در تشکیل بیوفیلم و مقاومت آنتی بیوتیکی در استافیلوکوکوس اورئوس های جدا شده از نمونه های ادراری بیمارستان امام خمینی (ره) تهران ميكروبیولوژی Microbiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 3.15in"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objective:</span></span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> <i>Staphylococcus aureus</i> causes problems in hospitals and it has emerged as a serious agent acquired from the environment in recent years. One of the capabilities of <i>S. aureus</i> is the formation of biofilms, in which bacteria can exchange antibiotic-resistance genes among themselves and increase the virulence of other strains of this species (<i>S. aureus</i>). A surface protein attached to the cell wall in S. aureus clumping factor A is a virulence factor in various staphylococcal infections.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 3.15in"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In this study, after the Urea Analysis (UA) test, the urea culture test was applied to the blood agar and Baird-Parker Agar culture media from the infectious urine samples in Imam Hospital, Tehran, to identify S. aureus isolates. Finally, a molecular method was used for the confirmation of identified isolates. The microliter plate method was performed to determine the biofilm formation ability<span style="color:red">. </span>The disk diffusion method was also used for profiling the antibiotic resistance of the isolates.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 3.15in"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In the results of this study, 45 out of 160 urinary clinical samples were positive for <i>S. aureu<b>s</b></i>, among which 42 isolates expressed the <i>clfA </i>gene. Moreover, 39 isolates had the ability to form biofilms in vitro. Among these 42 isolates, the highest (88%) and the lowest (16%) rates of antibiotic resistance were observed against penicillin and cefoxitin, respectively. Data analysis with SPSS software and chi-square indicated a significant relationship between gene expression and biofilm production with antibiotic resistance (P < 0.05). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 3.15in"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></b> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">The resistance of <i>S. aureus</i> bacteria is increasing strongly due to the repeated use of antibiotics such as beta-lactams, especially in respiratory infections and pharyngitis. Moreover, biofilm formation and virulence factors, such as <i>clfA</i> and <i>clfB</i>, cause concerns to the World Health Organization for treatment, especially for people with sepsis or toxemia.</span></span></span></span></span></span></span></div> <br> &nbsp; Staphylococcus aureus, clumping factors, coagulase, biofilm, microtitre plate, disk diffusion, virulence factors 242 251 http://jabs.fums.ac.ir/browse.php?a_code=A-10-623-3&slc_lang=en&sid=1 Leila Ghasem Azizi لیلا قاسم عزیزی arash.gh.az.1989@gmail.com 100319475328460027824 100319475328460027824 No Department of Microbiology, Faculty of Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه میکروبیولوژی، دانشکده علوم، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران Arash Ghasem Azizi آرش قاسم عزیزی dr.azizi86@gmail.com 100319475328460027825 100319475328460027825 Yes Department of Biology, Faculty of Science, Borujerd Branch, Islamic Azad University, Borujerd, Iran گروه زیست شناسی، دانشکده علوم، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران Behzad Hemati بهزاد همتی 100319475328460027826 100319475328460027826 No Department of Microbiology, Faculty of Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه میکروبیولوژی، دانشکده علوم، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران Monir Ebadi منیر عبادی 100319475328460027827 100319475328460027827 No Department of Microbiology, Faculty of Science, Karaj Branch, Islamic Azad University, Karaj, Iran گروه میکروبیولوژی، دانشکده علوم، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران