en بيوشيمي بالینی Biochemistry پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:67.5pt 6.5in"><span style="text-autospace:none"><span b="" style="font-family:" yagut=""><b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496"> <b>& Objective:</b> </span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Lead (Pb) is a major toxicant that has threatened human health for years. There is no safe level of exposure and</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> deposition of a small amount of Pb in the human body has a negative impact on an individual&#39;s health. D</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">espite recent reductions in its use,</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> it </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">has caused extensive environmental</span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">contamination as well</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">. In t</span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">he present study, </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">the</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> intralaboratory quality control assessment is reported as an initial step in lead determination by Graphite furnace atomic absorption spectrometry (GFAAS).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:67.5pt 6.5in"><span style="text-autospace:none"><span b="" style="font-family:" yagut=""><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials &</span></span></span></b> <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Methods:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Varian SpectrAA-220 with partition tube and deuterium background correction was used for the analysis. The evaluation quality control blood material was Seronorm trace elements whole blood (levels I &II).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:67.5pt 6.5in"><span style="text-autospace:none"><span b="" style="font-family:" yagut=""><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results:</span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The lowest method limit of detection was 0.74 &mu;g/dL, and based on the regression analysis, the lowest quantification concentration was 3.00 &mu;g/dL (CV = 4.6 %). In addition, obtained %CV for Seronorm (I) & Seronorm (II) was 7.3% & 5.4% respectively.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:67.5pt 6.5in"><span style="text-autospace:none"><span b="" style="font-family:" yagut=""><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> In recent years, several diverse technological advancements in blood lead level determination have occurred. At the same time, demands for blood lead (BPb) level determination by GFAAS has been increased by public health in order to identify children exposed to lead in the environment, and by occupational health to reduce excessive exposure in the lead industries. The obtained results indicated that for BPb analysis, the method described by GFAAS is still a reference routine analytical technique that requires less time and no matrix matched standard curve, allowing blood samples to be run against aqueous calibration standards which eliminates the need for standard additions. </span></span></span></span></span></span></span><br> &nbsp;</div> Lead Exposure- Graphite Furnace Atomic Absorption- Biomonitoring- Precision-Intralaboratory 131 137 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3240-1&slc_lang=en&sid=1 Mehri Aliasgharpour مهری علی اصغرپور mehri9@gmail.com 100319475328460027537 100319475328460027537 Yes Reference Health Laboratory, Ministry of Health & Medical Education, Tehran, Iran آزمایشگاه مرجع سلامت، وزارت بهداشت، درمان و آموزش پزشکی، تهران، ایران