en ويروس شناسي Virology پژوهشي Research <div style="text-align: justify;"><span style="line-height:normal"><span style="unicode-bidi:embed"><b><span style="color:#2f5496">Background & Objectives: </span></b>Papillomaviruses are found in many different types of infections and in a wide range of<b> </b>animals and humans. They can cause health problems, including benign and malignant tumors. In the present study, the association between human papillomavirus (HPV) infection and breast cancer (BC) in Iran was investigated.</span></span><br> <span style="line-height:normal"><span style="unicode-bidi:embed"><b><span style="color:#2f5496">Materials & Methods:</span></b> In this cross-sectional study, the presence of the HPV genome was investigated in BC-suspected tissues for the first time in Qom Province, Iran. A total of 400 samples (including 200 BC-suspected tissue samples and 200 blood samples of women without BC) were collected from women referred to two cancer-specific general hospitals. To determine the presence of the L1 gene of HPV in the collected samples, nested polymerase chain reaction (PCR) was performed. Then, HPV-positive samples were tested by PCR using high-risk specific HPV-16 and 18 primers.</span></span><br> <span style="line-height:normal"><span style="unicode-bidi:embed"><b><span style="color:#2f5496">Results:</span></b> Out of 200 BC-suspected tissue samples, 172 were malignant (in terms of pathology). Based on the nested PCR method, the L1 gene of HPV was detected in 12% (24/200) of the BC-suspected tissue samples and in 1.5% (3/200) of the blood samples from women without BC. The high-risk HPV genotypes (which were the predominant types) were present in 75% of the samples.</span></span><br> <span style="line-height:normal"><span style="unicode-bidi:embed"><b><span style="color:#2f5496">Conclusion:</span></b> The results of the current study show a high frequency of HPV-16 and 18 genotypes in human BC in Iranian women. This is almost certainly due to poor rates of HPV vaccination, and it is strongly recommended that health organizations (such as the World Health Organization [WHO]) ensure adequate coverage of highly effective HPV vaccination in Iran.</span></span><br> <br> &nbsp;</div> Human Papillomavirus, Breast cancer, Nested PCR 158 166 http://jabs.fums.ac.ir/browse.php?a_code=A-10-487-4&slc_lang=en&sid=1 Mohaddeseh Khalilian محدثه خلیلیان m.khalilian88@yahoo.com 100319475328460027498 100319475328460027498 No Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran گروه میکروبیولوژی و بیوتکنولوژی میکروبی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران، ایران Seyed Masoud Hosseini سید مسعود حسینی m.microbiology88@gmail.com 100319475328460027499 100319475328460027499 No Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran گروه میکروبیولوژی و بیوتکنولوژی میکروبی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران، ایران Hassan Vahidi Emami حسن وحیدی امامی h.vahidiemami@ut.ac.ir 100319475328460027500 100319475328460027500 No Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران Omid Madadgar امید مددگر madadgar@msu.edu 100319475328460027501 100319475328460027501 Yes Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI, USA گروه میکروبیولوژی و ژنتیک مولکولی، دانشگاه ایالتی میشیگان، ایست لنسینگ، MI، ایالات متحده آمریکا