en اثرات محافظتی رادیویی ملاتونین بر آسیب بافتی ناشی از تشعشع گاما در موش صحرایی بيوشيمي بالینی Biochemistry پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Background & Objective:</span></span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Gamma rays are widely used in medicine despite their harmful effects on health. Our study evaluated the protective effects of melatonin on kidney, heart, and liver tissues.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Materials & Methods:</span></span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Seventy-two adult male Wistar rats were categorized into nine groups.</span></span></span> <span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Groups 2 and 3 only received whole-body &gamma;-ray irradiation (WBI) (2.0 Gy), no melatonin,&nbsp; interval time (IT) 8hr and 24hr. Groups 4 and 5 received WBI (8.0 Gy, no melatonin, 8hr and 24hr). Groups 6 and 7 received melatonin at 60 minutes (</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">min</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">) before WBI(2.0 Gy). Groups 8 and 9 received melatonin before WBI(8.0 Gy). All&nbsp;the rats were sacrificed 8 or 24 h after the experiments for laboratory and histopathological analysis. Serum levels of Blood Urea Nitrogen(BUN), Creatinine (Cr), Lactate dehydrogenase (LDH), Potassium (K), Gamma-glutamyl Transferase (GGT), Serum Glutamic-Oxaloacetic Transaminase(SGOT), Serum Glutamic-Pyruvic Transaminase(SGPT), Alkaline Phosphatase(ALP), c-reactive protein(CRP), Troponine(TPO) &nbsp;and histological features of liver, heart and kidney tissues were assessed. Statistical analysis was performed by One-Way ANOVA. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Results: </span></span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Our data did not indicate significant differences in BUN, Cr, K, and CRP between groups with or without melatonin treatment (P>0.05); but differences were significant for LDH, GGT, SGOT, SGPT, ALP, and TPO (P<0.05). The results showed that radiation-induced histopathological effects on the liver, heart, and kidneys were mitigated in the groups six to nine. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:#2f5496">Conclusion:</span></span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">The existence of significant differences in serum levels of LDH, GGT, SGOT, SGPT, ALP, and TPO in groups(6 to 9) and amelioration of the histopathological effects of irradiation on the liver, heart, and kidneys in the groups six to nine showed that the melatonin(100 mg/kg) &nbsp;is able to protect the body in gamma-radiation(2.0 Gy and 8.0 Gy).</span></span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span><br> &nbsp; Melatonin, Oxidative stress, Whole-Body Irradiation, Histology. 102 115 http://jabs.fums.ac.ir/browse.php?a_code=A-10-3221-1&slc_lang=en&sid=1 Gholam Reza Bagheri غلامرضا باقری drbagherigr@gmail.com 100319475328460027526 100319475328460027526 No Department of Nutrition, School of Health, Zabol University of Medical Sciences, Zabol, Iran گروه تغذیه، دانشکده بهداشت، دانشگاه علوم پزشکی زابل، زابل، ایران Hamid Reza Ghaffari حمیدرضا غفاری hamidghaffary@yahoo.com 100319475328460027527 100319475328460027527 No Department of Basic Sciences, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran گروه علوم پایه، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران Majid Valizadeh مجید ولی زاده valizadehmph@gmail.com 100319475328460027528 100319475328460027528 No Department of Medical Physics, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran گروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران Hossein Ali Jahantigh حسینعلی جهان تیغ Hoseinjahantigh@yahoo.com 100319475328460027529 100319475328460027529 No Department of Pathology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran گروه آسیب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران Marzieh Poursamimi مرضیه پورصمیمی Poursamimimarzieh@gmail.com 100319475328460027530 100319475328460027530 No Department of Clinical Psychology, Faculty of Humanities, Sirjan Branch, Islamic Azad University, Sirjan, Iran گروه روانشناسی بالینی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی، سیرجان، ایران Hosein Shahriari حسین شهریاری hosseinshahriari@yahoo.com 100319475328460027531 100319475328460027531 No Clinical Immunology Research Center, Zahedan University of medical sciences, Zahedan, Iran مرکز تحقیقات ایمونولوژی بالینی، دانشگاه علوم پزشکی زاهدان، زاهدان، ایران Javad Poursamimi جواد پورصمیمی Javadpoursamimi@gmail.com 100319475328460027532 100319475328460027532 Yes Department of Immunology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران