en بررسی میزان آراشیدونیک اسید، ایکوزاپنتانوئیک اسید و آمیلوئید آ در سرم زنان مبتلا به سندروم تخمدان پلی کیستیک زنان و زايمان Gynecology & Obstetrics پژوهشي Research <div style="text-align: justify;"><span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:#2f5496">Background & Objective:</span></span></b> <span style="font-size:10.0pt">PCOS is a multifactorial disorder characterized by inflammation caused by unknown reasons. This study aimed to investigate the Arachidonic acid (AA), eicosapentaenoic acid (EPA) levels and Serum amyloid A (SAA) </span><span style="font-size:10.0pt">protein and gene expression of </span><span style="font-size:10.0pt"><span style="color:black">follicular fluid </span></span><span style="font-size:10.0pt">in women with PCOS.<span style="color:black"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:#2f5496">Materials & Methods: </span></span></b><span style="font-size:10.0pt"><span style="color:black">The study included 30 PCOS and 30 healthy women. The individuals were selected after clinical examination and the inclusion criteria. During the puncture and after removing oocytes, follicular fluids were collected from individuals. The SAA </span></span><span style="font-size:10.0pt">protein <span style="color:black">concentration was determined by the ELISA method. Arachidonic acid, eicosapentaenoic acid levels were determined using GC method and the statistical software SPSS 16,</span></span><span style="font-size:10.0pt"><span style="color:black"> and the p value less than 0.05 was considered as significant.</span></span><span style="font-size:10.0pt"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:#2f5496">Results:</span></span></b> <span style="font-size:10.0pt">There was a significant difference in metaphase II oocytes (M2) (p =0.001), number of Embryo (p =0.014), Estradiol (p =0.025), FSH (0.040), LH (p =0.035) and Prolactin (p =0.023) levels between two groups. </span><span style="font-size:10.0pt">The concentration of SAA protein level and gene expression shows a significant increase in the patient group compared to the control group (p <0.05).</span><span style="font-size:10.0pt"><span style="color:black"> The results showed that AA level was significantly increased in the patient group (284.12 ng/mL) compared to the control group (176.23 ng/mL) </span></span><span style="font-size:10.0pt">(p =0.001) while, </span><span style="font-size:10.0pt"><span style="color:black">there was a significant decrease </span></span><span style="font-size:10.0pt">(p =0.001)</span><span style="font-size:10.0pt"><span style="color:black"> in EPA level in the patient group (24.56 ng/mL) compared to the control group (33.48 ng/mL). </span></span><span style="font-size:10.0pt"></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="text-autospace:none"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:#2f5496">Conclusion:</span></span></b> <span style="font-size:10.0pt">It appears that higher EPA levels and lower follicular fluid AA concentrations reduce SAA levels and thus may increase follicular maturation in women with PCOS.</span></span></span></span></span></div> PCOS, Follicular maturation, Serum amyloid A, Fatty Acids 29 39 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1810-6&slc_lang=en&sid=1 Sahar Mazloomi سحر مظلومی saharmazloomi1001@yahoo.com 100319475328460027644 100319475328460027644 No Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران Iraj khodadadi ایرج خدادای ikhodadadi@yahoo.com 100319475328460027645 100319475328460027645 No Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران Marzieh Farimani مرضیه فریمانی m.farimani@yahoo.com 100319475328460027646 100319475328460027646 No Department of Obstetrics and Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران Marzieh Ghorbani مرضیه قربانی m.ghorbani@yahoo.com 100319475328460027647 100319475328460027647 No Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران Gholamreza Shafiee غلامرضا شفیعی g.r_shafiee@yahoo.com 4051460312 100319475328460027648 Yes Department of Clinical Biochemistry, School of Medicine, Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran گروه بیوشیمی بالینی، دانشکده پزشکی، مرکز تحقیقات بهداشت تغذیه، دانشگاه علوم پزشکی همدان، همدان، ایران