en تاثیر سن مادر بر آنوپلوئیدی کروموزومی، کیفیت بلاستوسیت و پیامدهای بارداری در سیکل های تزریق داخل سیتوپلاسمی اسپرم جنين شناسي Embryology پژوهشي Research <div> <div> <div id="_com_1" uage="JavaScript"> <div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> Assisted reproduction techniques and preimplantation genetic testing (PGT) for aneuploidies help infertile couples achieve a healthy live birth. The objective of this study was to investigate if there is a correlation between chromosomal aneuploidy and maternal age.<br> <strong><em>Materials & Methods:</em></strong> We used 277 embryos with 6-8 cells and graded A derived from intracytoplasmic sperm injection cycles. There were two subgroups: fresh<span dir="RTL">-</span>PGT cycles (n = 38) and Frozen Embryo Transfer (FET) cycles (n = 38). The PGT cycles results were evaluated by fluorescence in situ hybridization and polymerase chain reaction assays. Couples (n = 76) with PGT on the third day were classified into four maternal age groups: &le;35 years (n = 35), 36-40 years (n = 24), 41-45 years (n = 11), and &ge;46 years (n = 6). The rate of aneuploidy, blastocyst quality, and pregnancy was assessed in FET and fresh-PGT cycle.<br> <strong><em>Results:</em></strong> Aneuploidy rate in 13, 18, 21, X, and Y chromosomes in FET and fresh-PGT cycles are not significantly related to maternal age (p = 0.1). Significant differences were found in a decreased chemical pregnancy (p = 0.001), clinical pregnancy (p = 0.001), ongoing pregnancy (p = 0.001), and live birth rate (p = 0.001) among both groups. Decreased blastocyst rate increased maternal age in fresh PGT and FET PGT (p = 0.02). Early and clinical pregnancy loss was not related significantly to maternal age.<br> <strong><em>Conclusion:</em></strong> After evaluating cycle characteristics, an association was found between maternal age and declined pregnancy outcomes in embryos. There was a significant relationship between increasing female age with decreasing blastocyst rate.<span dir="RTL"></span><br> <span dir="RTL"></span></div> <div> <div><div id="_com_4" uage="JavaScript"></div></div> </div> </div> </div> <div></div></div> <span dir="RTL"></span><span dir="RTL"></span> Blastocyst Quality, Pregnancy Outcomes, Intracytoplasmic Sperm Injection Cycles, Fluorescence in Situ Hybridization, Preimplantation Genetic Testing 4107 4117 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2390-3&slc_lang=en&sid=1 Shahrzad Aghajani شهرزاد آقاجانی shahrzadaghajani1987@gmail.com 100319475328460027879 100319475328460027879 No Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد رشت ، رشت، ایران Ali Salehzadeh علی صالح زاده salehzadeh@iaurasht.ac.ir 100319475328460027880 100319475328460027880 Yes Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد رشت ، رشت، ایران Fatemeh Ghasemiyan فاطمه قاسمیان ghasemiyan@guilan.ac.ir 100319475328460027881 100319475328460027881 No Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران Marziyeh Mehrafza مرضیه مهرافزا marzieh.mehrafza@gmail.com 100319475328460027882 100319475328460027882 No Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran مرکز تحقیقات باروری مهر، دانشگاه علوم پزشکی گیلان، رشت، ایران Ahmad Hosseini احمد حسینی prof_hosseini@yahoo.com 100319475328460027883 100319475328460027883 No Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran مرکز تحقیقات باروری مهر، دانشگاه علوم پزشکی گیلان، رشت، ایران