en شناسایی اپی توپ های b-cell در راستای تعیین نقاط ایمونولوزیک موثر جهت تولید واکسن ايمونولوژي Immunology پژوهشي Research <strong><em>Background & Objective</em></strong><strong>:</strong> The most common cause of milk production loss in cattle and water buffalo is bovine ephemeral fever (BEF). Previous cases have been reported in Iran&#39;s south regions, with a low mortality rate. As a result, studying BEFV and identifying ideal epitopes for further developing diagnostic paths is important.<br> <strong><em>Materials & Methods:</em></strong> To investigate BEFV N protein epitopes, we collected samples, extracted and sequenced DNA, and then used the ExPaSy translate method to deduce the amino acid sequence. Various immunoinformatics techniques were used to analyze physical/chemical properties, secondary structure of protein sequences, membrane topology, antigenic property, and 3D structure. BCPRED and the DiscoTope server, respectively, predicted linear and discontinuous epitopes of BEFV N protein. Finally, the PatchDock server was used to dock peptides and antibodies.<br> <strong><em>Result:</em></strong> Three linear epitopes and sixteen discontinuous epitopic positions were discovered. Furthermore, molecular docking between epitopes and low-binding-energy antibodies revealed that they have easy access to the immune system.<br> <strong><em>Conclusion</em></strong><strong>:</strong> In this study, bioinformatics techniques were used to predict epitopes of the BEFV N protein for further developing BEFV diagnostic paths. Furthermore, experimental validation is needed for these epitopes.<br> &nbsp; Epitopes, Immunoinformatics, Docking 79 87 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2968-1&slc_lang=en&sid=1 Shima Molazadeh شیما ملازاده shima.molazadeh@gmail.com 100319475328460025725 100319475328460025725 No Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Hadi Keivanfar هادی کیوانفر hadi.99keivanfar@gmail.com 100319475328460025726 100319475328460025726 Yes Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه پاتوبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Mehran Bakhshesh مهران بخشش mehran.99bakhshesh@gmail.com 100319475328460025727 100319475328460025727 No Department of Animal Virology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Organization (AREEO), Karaj, Iran گروه ویروس شناسی دامی، مؤسسه تحقیقات واکسن و سرم سازی رازی، تحقیقات، آموزش و سازمان کشاورزی (AREEO)، کرج، ایران Gholamreza Nikbakht Brujeni غلامرضا نیکبخت بروجنی gholamreza.99nikbakht@gmail.com 100319475328460025728 100319475328460025728 No Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران