en ارزیابی ریزنشت و زیست سازگاری یونیورسال ادهزیو حاوی نانوذرات دی اکسید تیتانیوم: بررسی آزمایشگاهی دندانپزشكي Dentistry پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objective</em></strong><em>:</em> Although dental composites have undergone a high level of development in recent years, there are still difficulties including microleakage. The incorporation of nanoparticles (NPs) in dental materials can produce antibacterial effects, but the effect of TiO₂ NPs on microleakage has not yet been investigated. The present study aimed to determine the effect of TiO₂ NPs incorporation in the universal single bond and pretreatment with them on microleakage of clV cavities using both total-etch and self-etch approaches.<br> <strong><em>Materials & Methods</em></strong><em>:</em> Standard clV cavities were prepared on the buccal surfaces of the sixty non-carious human molar teeth. The restoration of samples was carried out with 6 different methods and randomly divided into six groups of ten subjects. Microleakage in both gingival and occlusal margins was determined after placement in 2% basic fuschin solution for 24h. Biocompatibility of a universal single bond was evaluated by MTT assay.<br> <strong><em>Results</em></strong><em>:</em> In both occlusal and gingival margins in all groups, the application of TiO₂ NPs was better than no application. We observed the relatively low cytotoxic effect of TiO₂ NPs incorporated in the universal adhesive on NIH-3T3 cell viability.<br> <strong><em>Conclusion</em></strong><em>:</em> Regarding the limitations of the present investigation, using TiO₂ NPs with both total-etch and self-etch approaches to universal single bond&nbsp;may lead to a decrease in the microleakage of resin composite restorations.<br> &nbsp;</div> TiO2 NPs, Microleakage, Universal Bond, NIH-3T3 4030 4039 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2959-1&slc_lang=en&sid=1 Fatemeh koohpeima فاطمه کوهپیما koohpeima.f@gmail.com 100319475328460026433 100319475328460026433 No Department of Operative Dentistry, Biomaterial Research Center, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran گروه دندانپزشکی جراحی، مرکز تحقیقات بیومتریال، دانشکده دندانپزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران Mohammad Javad Mokhtari محمدجواد مختاری Mj.mokhtari@gmail.com 100319475328460026434 100319475328460026434 Yes Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran گروه زیست شناسی، واحد زرقان، دانشگاه آزاد اسلامی، زرقان، ایران Marzieh Salari مرضیه سالاری dr.m.salari73@gmail.com 100319475328460026435 100319475328460026435 No Department of Operative Dentistry, Biomaterial Research Center, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran گروه دندانپزشکی جراحی، مرکز تحقیقات بیومتریال، دانشکده دندانپزشکی، دانشگاه علوم پزشکی شیراز، شیراز، ایران