Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
JABS
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2228-5105
2783-1523
8
7
14
8888
13
en
jalali
1401
1
1
gregorian
2022
4
1
12
2
online
1
fulltext
en
The Protective Effect of Curcumin on the Proliferation and Colonization of Spermatogonial Stem Cells in Gamma-Irradiated Rats
گیاهان دارویی
Pharmaceutical Plants
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objective:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> One of the side effects of radiotherapy can be damage to spermatogonial stem cells that ‎may lead to spermatogenesis disorders and sterility. Protective effects of curcumin on normal cells ‎against radiotherapy side effects have already been shown. In the current study, the protective effects of curcumin ‎on the spermatogonial stem cells against gamma radiation were ‎evaluated.‎</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials & Methods:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> This study was done on 50 adult rats in 10 experimental groups. Four groups were ‎injected 0, 25, 50, or 100mg/kg of curcumin in 1ml olive oil for 15 days intraperitoneally, then exposed to radiation ‎at 2 Gy on the next day. Also, four groups were treated like above but without radiation; and two groups as control with and without radiation. The day after radiation, all of the rats were euthanatized, their testes were removed, and they underwent enzymatic digestion to co-culture spermatogonial stem cells. After 12 days, the colonization of spermatogonial stem cells was assessed. ‎</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> There was a significant decrease in the colonization of spermatogonial stem cell proliferation ‎in groups that had taken radiation but not curcumin. There was a significant increase ‎ in the colonization of spermatogonial stem cells in the group which had taken radiation whit maximum curcumin compared with the other irradiated groups and ‎was similar to non-irradiated control animals. Colonization of spermatogonial stem cells in non-irradiated animals treated with curcumin had increased compared with control groups.‎</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Injection of curcumin can protect spermatogonial stem cells against ‎radiation. Thus, curcumin may prevent sterility in men who undergo radiotherapy. ‎</span></span></span></span></span><br>
</div>
Curcumin, Radiation therapy, Radiation protective agents, Spermatogonial stem cells, Sterility
194
202
http://jabs.fums.ac.ir/browse.php?a_code=A-10-2942-1&slc_lang=en&sid=1
Sareh
Azarmi
ساره
آزرمی
sareh.azarmi@yahoo.com
100319475328460026090
100319475328460026090
No
Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
khosro
Hoseini pajooh
خسرو
حسینی پژوه
pajooh@irost.ir
0043390560
100319475328460026091
Yes
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
گروه بیوتکنولوژی، سازمان تحقیقات علم و فناوری ایران (IROST)، تهران، ایران
Parviz
Tajik
پرویز
تاجیک
ptajik@ut.ac.ir
100319475328460026092
100319475328460026092
No
Department of Theriogenology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
گروه تریوژنولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
Marziyeh
Lashkari
مرضیه
لشکری
m-lashkari@sina.tums.ac.ir
100319475328460026093
100319475328460026093
No
Department of Radiotherapy, Tehran University of Medical Sciences, Tehran, Iran
گروه رادیوتراپی، دانشگاه علوم پزشکی تهران، تهران، ایران