Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
JABS
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2228-5105
2783-1523
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8888
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jalali
1400
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gregorian
2021
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fulltext
en
تشخیص کمی سالمونلا تیفی با تکنیک Real-Time Loop-Mediated Isothermal Amplification براساس ژن ViaB
Real-Time Loop-Mediated Isothermal Amplification (LAMP) Method for Quantitative Salmonella Typhi Detection Based on ViaB Gene
ميكروبیولوژی
Microbiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background & Objective:</strong> The <em>Salmonella</em> origin diseases is wide which could be from gastroenteritis, enteric fever, bacteraemia and focal infection. The infection of salmonella usually comes from the consumption of the contaminated food or water with animal or human excrement. This study aimed to design a Quantitative-LAMP method to real-time detection of <em>Salmonella enterica</em> serovar Typhi (<em>S. </em>Typhi), the causative agent of Typhoid fever.<br>
<strong>Materials & Methods:</strong> A new LAMP primer set specifically was designed based on <em>ViaB</em> gene and used for detection of <em>S. Typhi.</em> To evaluate the analytical specificity, the genome of some Salmonella-related and non-related bacteria were subjected to the <em>S. </em>Typhi LAMP assay. Also, the analytical sensitivity or limit of detection of the assay was evaluated. Furthermore, in the experiment, turbidity in tubes was assessed by a turbidimeter and then a standard curve was depicted by plotting time threshold values against the log of <em>ViaB</em> gene copy number. With this standard curve, we could use the method to make a quantitative method</div>
<strong>Results:</strong> The <em>Salmonella</em> <em>Typhi</em> LAMP assay specifically assessed the <em>ViaB</em> gene. The analytical sensitivity of the assay when using agarose gel electrophoresis or amplification plot obtained from Loop amp real-time turbidimeter system was 0.28 fg whereas with direct observation of fluorescent color change to evaluate amplification was 2.8 fg. So, the lower limit of detection of the assay when applying the revealing methods was ~1 and 8 copies of the <em>ViaB</em> gene respectively. <br>
<strong>Conclusions:</strong> The <em>Salmonella</em> <em>Typhi</em> LAMP assay is a simple and accurate tool to detect the causative agent of Typhoid fever that may designate to apply in clinical laboratories
LAMP, Salmonella Typhi, Typhoid fever, Specificity and Sensitivity
3831
3838
http://jabs.fums.ac.ir/browse.php?a_code=A-10-2134-4&slc_lang=en&sid=1
Mahdi
Sohrab
مهدی
سهراب
100319475328460026350
100319475328460026350
No
Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد قم، قم، ایران
Keivan
Majidzadeh
کیوان
مجیدزاده
100319475328460026351
100319475328460026351
No
Breast Cancer Research Center (BCRC), Academic Center for Education, Culture & Research (ACECR), Tehran, Iran
مرکز تحقیقات سرطان سینه، جهاد دانشگاهی تهران، تهران، ایران
Abbas
Morovvati
عباس
مروتی
Abbasmorovvati@gmail.com
100319475328460026352
100319475328460026352
No
Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
گروه میکروبیولوژی، دانشگاه آزاد اسلامی واحد قم، قم، ایران
Mohammad
Soleimani
محمد
سلیمانی
Soleimanidor@yahoo.com
100319475328460026353
100319475328460026353
Yes
Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
گروه میکروبیولوژی، دانشگاه علوم پزشکی ارتش، تهران، ایران