en بررسی محتوای فنولی، قدرت آنتی اکسیدانی و اثرات سیتوتوکسیسیتی عصاره جلبک‌های دریایی Ulva lactuca و Hypnea musiformis بر رده‌ سلولی MDA-MB-468 سرطان سینه گیاهان دارویی Pharmaceutical Plants پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> Breast cancer is one of the leading causes of death among women around the world. Attempts to find alternative therapies continue due to therapeutic resistance, side effects, and the high cost of conventional treatments.<br> <strong><em>Materials & Methods:</em></strong> Hydro-methanolic extract of <em>Ulva lactuca</em> and <em>Hypnea musiformis</em> marine algae was prepared by maceration method. The phenolic content of these extracts was compared using the standard Folin-Ciocateu method and the antioxidant capacity was evaluated by the FRAP method. The cytotoxic effects of algae were compared using MTT assay and morphological changes were evaluated by an inverted microscope.<br> <strong><em>Results:</em></strong> The phenolic content of <em>Hypnea musiformis</em> extract (12.46 &plusmn; 1.19 &micro;g GAE/mg, P<0.0001), and its antioxidant activity (203.03 &plusmn; 27.87 &micro;mol Fe²⁺/g, P= 0.007), was significantly higher than that of the alga <em>Ulva lactuca</em>. The cytotoxic effects of <em>Hypnea musiformis</em> extract were concentration and time dependent and had severe morphological changes on the cells. Algae extract of <em>Hypnea musiformis</em> (1000 &micro;g/ml) inhibited 84.79 &plusmn; 4.66 of % cell proliferation after 72 hours of treatment. The IC50 value of cytotoxic effect of <em>Hypnea musiformis</em> and <em>Ulva lactuca</em> on MDA-MB-468 was respectively 701.2 and >1000 &micro;g/ml after 72h incubation.<br> <strong><em>Conclusions:</em></strong> <em>Hypnea musiformis</em> had higher cytotoxic effects than <em>Ulva lactuca</em> on MDA-MB-468 cells, probably due to its higher phenolic content and antioxidant capacity. Therefore, it appears that a <em>Hypnea musiformis</em> alga is a better option to continue research on drug discovery of anticancer compounds.</div> Algae (Ulva lactuca, Hypnea musiformis), Antioxidant, cytotoxic effects, Breast cancer 3921 3928 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2629-1&slc_lang=en&sid=1 Alireza Moulazadeh علیرضا مولازاده immunomoula@gmail.com 100319475328460026388 100319475328460026388 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری های غیرواگیردار، دانشگاه علوم پزشکی فسا، فسا، ایران Razieh Ranjbar راضیه رنجبر ranjbarrazie@gmail.com 100319475328460026389 100319475328460026389 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran مرکز تحقیقات بیماری های غیرواگیردار، دانشگاه علوم پزشکی فسا، فسا، ایران Amin Dakhili Ardestani امین دخیلی اردستانی amindakhili114@gmail.com 100319475328460026390 100319475328460026390 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Aref Keshavarzi عارف کشاورزی keshavarziaref@yahoo.com 100319475328460026391 100319475328460026391 No Fars blood transfusion organization research center, Shiraz, Iran مرکز تحقیقات سازمان انتقال خون فارس، شیراز، ایران Fatemeh Karimzadeh فاطمه کریم زاده fatemehkarimzadeh95@gmail.com 100319475328460026392 100319475328460026392 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Maryam Rahnavard مریم راهنورد maryam.shz.r@gmail.com 100319475328460026393 100319475328460026393 No Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی فسا، فسا، ایران Zahra Zarei Jeliani زهرا زارعی جلیانی 100319475328460026394 100319475328460026394 No Department of Marine Biology, University of Hormozgan, Bandar Abbas, Iran گروه زیست شناسی دریایی، دانشگاه هرمزگان، بندرعباس، ایران Sohrab Najafipour سهراب نجفی پور resimmuno@gmail.com 100319475328460026395 100319475328460026395 Yes Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran گروه میکروب شناسی، دانشگاه علوم پزشکی فسا، فسا، ایران