en تاثیر ان-استیل سیستئین بر سطح بیان ژن FAS در بافت بیضه موش های صحرایی بالغ تیمار شده با آکریل آمید سم شناسي Toxicology پژوهشي Research <div style="text-align: justify;">&nbsp;<strong><em>Background & Objectives:</em></strong> Acrylamide (ACR) is a chemical with toxic effects on various body tissues. The present study was conducted to investigate the antioxidant effect of N-acetylcysteine (NAC) on the level of testicular apoptosis in acrylamide-treated adult rats.<br> <strong><em>Material & Methods:</em></strong> Thirty-six adult male Wistar rats were randomly divided into 6 equal groups. The intact control group was without treatment, the positive control group (PC) received 50 mg/kg ACR by oral gavage, the negative control group (NC) received 40 mg/kg NAC intraperitoneally, the animals in experimental groups of 1 (EXP1), 2 (EXP2) and 3 (EXP3) received 10, 20 and 40 mg/kg NAC intraperitoneally, respectively, and then all groups received 50 mg/kg acrylamide by oral gavage. The treatment period in all groups was 28 days. At the end of the study, FAS mRNA expression level was measured by real-time PCR and testicular tissue was evaluated histopathologically.<br> <strong><em>Results:</em></strong> The PC group showed a significant increase in FAS gene expression level (p<0.05) and spermatogenic degradation compared to the intact control and NC groups. The EXP1 and EXP2 groups showed decrease in FAS gene expression level (p˃0.05) and spermatogenesis improvement in a dose-dependent manner while the EXP3 group exhibited a significant decrease in FAS gene expression level (p˂0.05) and complete spermatogenesis recovery compared to the PC group.<br> <strong><em>Conclusion:</em></strong> The findings indicate that ACR increases apoptosis and destroys spermatogenesis by increasing FAS gene expression levels. In contrast, at the maximum dose (40 mg/kg), NAC could inhibit ACR-induced apoptosis by reducing FAS gene expression and improves spermatogenesis in rats.<br> &nbsp;<br> &nbsp;</div> Apoptosis, N-acetylcysteine, Acrylamide, Testis, Rat 3848 3856 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2651-2&slc_lang=en&sid=1 Elham Shahrzad الهام شهرزاد el.shahrzad20@gmail.com 100319475328460026359 100319475328460026359 No Department of Biology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran گروه زیست شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران Mehrdad Shariati مهرداد شریعتی mehrdadshariati@kau.ac.ir 100319475328460026360 100319475328460026360 Yes Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran گروه زیست شناسی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران Sirous Naeimi سیروس نعیمی naeimi81@yahoo.com 100319475328460026361 100319475328460026361 No Department of Genetics, Kazerun Branch, Islamic Azad University, Kazerun, Iran گروه ژنتیک، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران Mohammad Amin Edalatmanesh محمد امین عدالت منش amin_edalatmanesh@yahoo.com 100319475328460026362 100319475328460026362 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran گروه زیست شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران