fa بررسی اثر سمیت متامفتامین بر سلول‌های بنیادین مزانشیمی مشتق شده از بافت چربی موش‌های صحرایی فيزيولوژي Physiology پژوهشي Research <pre style="text-align: justify;"> <strong style="font-family: Arial;"><em>Background & Objective:</em></strong><span style="font-family: Arial;"> Methamphetamine is a hallucinogenic and addictive substance that is widely used worldwide. Cellular toxicity in various cells, especially in nerve cells, is one of the reported effects of this psychoactive substance. Therefore, this study aimed to investigate the effect of methamphetamine cytotoxicity on adult mesenchymal stem cells</span> </pre> <pre style="text-align: justify;"> <span style="font-family:Arial;"><strong><em>Materials & Methods: </em></strong>In this experimental study, mesenchymal stem cells were extracted from the adipose tissue of adult male rats of the Wistar breed and after culturing, their being mesenchymal was confirmed by flowcytometry method using CD34-RPE, CD90-RPE and CD105-RPE conjugate antibodies. In the third passage of cell culture, the effect of methamphetamine toxicity at a concentration of 0.6 mmol during 1 to 7 days on the growth process of these cells was investigated by MTT test.</span></pre> <pre style="text-align: justify;"> <span style="font-family:Arial;"><strong><em>Results:</em></strong> The cells detached from the adipose tissue were completely adhered to the floor of the flask 24 hours after being transferred to the cell culture flask. The results of <a name="_Hlk41632780">flowcytometry </a>showed that the expression of negative endothelial surface marker (CD34) and the expression of mesenchymal cell markers (CD90, CD105) were positive, which confirmed the basic identity of the extracted cells. The results of the MTT test also showed a significant reduction in the growth of cells treated with 0.6 mmol methamphetamine compared with the control group at p <0.05.</span></pre> <div style="text-align: justify;"><span style="font-family:Arial;"><strong><em>Conclusion:</em></strong> Cells isolated from adipose tissue are the basic mesenchymal type in which methamphetamine can induce the effects of cell toxicity and inhibit growth.<br> &nbsp;</span></div> Mesenchymal stem cells, adipose tissue, methamphetamine, rat 2665 2672 http://jabs.fums.ac.ir/browse.php?a_code=A-10-72-8&slc_lang=fa&sid=1 Fariba Jafari Shibani فریبا جعفری شیبانی fjafari3@gmail.com 100319475328460023870 100319475328460023870 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran گروه زیست‌شناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران Seyyed Ebrahim Hosseini سید ابراهیم حسینی ebrahim.hossini@yahoo.com 100319475328460023871 100319475328460023871 Yes Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran گروه زیست شناسی،واحد شیراز،دانشگاه آزاد اسلامی،شیراز،ایرانث Davood Mehrabani داوود مهربانی davood_mehrabani@yahoo.com 100319475328460023872 100319475328460023872 No Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, مرکز تحقیقات سوختگی و زخم، دانشگاه علوم پزشکی شیراز، شیراز، ایران