fa اثرات تیمول در مدل تجربی روماتوئید آرتریت ايمونولوژي Immunology پژوهشي Research <strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مقدمه و هدف</span></span></span></strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">: امروزه توجه زیادی به ترکیبات گیاهی باقابلیت درمانی شده است. هدف از مطالعه حاضر ارزیابی اثرات تیمول در مدل حیوانی روماتوئید آرتریت</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">(</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">RA</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">) است.</span></span></span><strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:13.0pt;"></span></span></span></span></strong><br> <strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مواد و روش</span></span></span></strong><strong><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></strong><strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ها:</span></span></span></strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> بیماری </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">RA</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> با استفاده از تزریق ادجوانت فروند کامل در بالشتک کف پای رت&shy;های ویستار القاء شد. سپس رت</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ها به‌صورت تصادفی در سه گروه بدون تیمار، تیمار شده با</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تیمول (</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">mg/kg</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">100-خوراکی) و یا درمان شده با پردنیزولون (</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">mg/kg</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">10-خوراکی) قرار گرفتند. کلیه درمان</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ها از روز پنجم پس از القاء شروع شد. تغییر قطر ناحیه </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">dorso-plantar</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> دست و پای هر رت</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تا روز 23 پس از القاء، یک روز در میان ثبت شد.</span></span></span><br> <strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتایج</span></span></span></strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">:</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میزان ادم و تورم کف پا در هر دو گروه رت</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های مبتلا و درمان شده با تیمول و یا پردنیزولون به‌صورت تقریباً برابر نسبت به گروه شاهد</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کاهش یافت. سطح نیتریک اکسید و آنزیم میلوپراکسیداز سرمی، هم‌زمان با شدت تکثیر لنفوسیت</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های طحالی و میزان تولید سایتوکاین</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">IL-1</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">IL-6</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> در گروه&shy;های درمانی نسبت به گروه شاهد کاهش معنی‌داری یافت. میزان کاهش نیتریک اکسید سرمی در گروه دریافت‌کننده تیمول نسبت به گروه</span></span></span> <span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دریافت‌کننده پردنیزولون بیشتر بود. در عوض پردنیزولون موجب کاهش بیشتری در سطح سایتوکاین</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">IL-1</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">IL-6</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> نسبت به رت</span></span></span><span dir="LTR" style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های مبتلا و دریافت‌کننده تیمول شد.</span></span></span><br> <strong><span style="letter-spacing:-.2pt;layout-grid-mode:line;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتیجه‌گیری</span></span></span></strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">: استفاده از تیمول ممکن است که به‌عنوان یک استراتژی سودمند در کنترل بیماری </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">RA</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> مطرح گردد.</span></span> <strong>B<em>Background & Objectives:</em></strong> Nowadays, attention to natural products with therapeutic potential has been increased. This study was carried out to investigate the effect of Thymol on the animal model of Rheumatoid arthritis (RA).<span dir="RTL"></span><br> <strong><em>Materials & Methods:</em></strong> RA was induced by injection of complete Freund&#39;s adjuvant into the footpad of Wistar rats. Then, rats were allocated in 3 groups: treated with Thymol (100 mg/kg-orally), treated with Prednisolone (100 mg/kg-orally) and un-treated group. All treatments were initiated at the 5^th day after induction. The change in the dorso-plantar diameter of hands and legs of each rat were recorded every other day until 23 days after induction.<br> <strong><em>Results</em></strong><strong><em>:</em></strong> The edema and swelling of the soles of the feet of RA rats that received Thymol or prednisolone, were significantly decreased in a similar manner compared to RA rat without treatment. The serum levels of nitric oxide and myeloperoxidase correlated with the proliferation of spleen lymphocytes and the levels of IL-1 and IL-6 production were significantly decreased in treatment groups compared to control group rats. The level of decrease in the level of serum nitric oxide was higher in the thymol group compared to the prednisolone-induced group. Conversely, prednisone caused more decrease in the levels of IL-1 and IL-6 cytokines compared to RA rats that received thymol.<br> <strong><em>Conclusion</em></strong><strong><em>:</em></strong> Thymol may be considered as a useful strategy to control RA disease. تیمول, روماتوئید آرتریت, پاسخ‌های ایمنی Thymol, Rheumatoid Arthritis, Immunity Responses 890 900 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1722-1&slc_lang=fa&sid=1 khadije golbahari خدیجه گلبهاری kh.golbahari@yahoo.com 2280533774 100319475328460018953 No Urmia University گروه میکروب شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران Meysam Abtahi Froushani میثم ابطحی فروشانی meysamabtahi@hotmail.com 100319475328460018954 100319475328460018954 Yes Urmia University گروه میکروب شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران