fa بررسی محتوای فنل، فلاونوئید کل و عملکرد ضد اکسیدانی گیاه نادر سوسن چلچراغ(Lilium ledebourii (Baker) Boiss) در شمال ایران گیاهان دارویی Pharmaceutical Plants پژوهشي Research <p dir="RTL"><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">سوسن چلچراغ بانام علمی </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">Lilium ledebourii</span></span></span></em><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;"> (Baker) Boiss</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> تنها گونه گیاهی طبیعی و ملی کشور با پراکنش محدود در مناطق کوهستانی جنگل هیرکانی است که بسیاری از جنبه&shy;های اکولوژیکی، زینتی و دارویی آن موردبررسی قرار نگرفته است.</span></span><strong><span dir="LTR"><span style="color:red;"><span style="font-family:times new roman,serif;"><span style="font-size:13.0pt;"></span></span></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مواد و روش‌ها:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> بعد از تهیه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره اتانولی</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">روش</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">خیساندن، فنل</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کل</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">روش</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فولین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">سیکالتو،</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فلاونوئید</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کل به</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">روش</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آلومینیوم</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلراید و فعالیت</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ضد اکسیدانی</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره نمونه&shy;های</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">برگ و ساقه،</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">روش</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;"> DPPH </span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اندازه&shy;گیری</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">.</span></span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> برای</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تجزیه‌وتحلیل</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آماری</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تجزیه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">واریانس</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">برای</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مقایسه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میانگین&shy;ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آزمون</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دانکن</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">استفاده</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شد.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتایج: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میزان</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فنل</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کل</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره اتانولی اندام</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های هوایی سوسن در چهار رویشگاه بین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">59/6</span></span><span style="font-size:10.0pt;">&plusmn;</span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">65/43</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">21/9</span></span><span style="font-size:10.0pt;">&plusmn;</span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">14/66</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میلی&shy;گرم گالیک اسید در گرم عصاره و</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ظرفیت ضد اکسیدانی آن بین 51 تا 90 درصد متغیر بود، بیشترین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میزان فنل کل و نیز فعالیت</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ضد اکسیدانی</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اندام&shy;های هوایی</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">منطقه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلاردشت (بالاترین ارتفاع)</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کمترین میزان</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">رستم آباد</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">(پایین&shy;ترین ارتفاع)</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">یافت</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شد. محتوای</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فلاونوئید</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره اتانولی اندام&shy;های هوایی سوسن چلچراغ نیز بین 91/7</span></span><span style="font-size:10.0pt;">&plusmn;</span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">41/33 و 21/1</span></span><span style="font-size:10.0pt;">&plusmn;</span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">72/43 میلی&shy;گرم</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کوئرستین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">گرم</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عصاره بود که بالاترین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میزان آن</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">منطقه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اسالم</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کمترین میزان</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آن‌ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">منطقه</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نمین</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مشاهده</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">.</span></span></span><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"></span></span></strong><br> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتیجه&shy;گیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> از نتایج این تحقیق می&shy;توان استنتاج نمود که در مقایسه با بسیاری از گونه&shy;های دارویی دیگر، سوسن چلچراغ به لحاظ میزان ضد اکسیدان، از ظرفیت نسبتاً خوبی برخوردار است که این میزان بسته به محل رویش سوسن چلچراغ متفاوت بوده و همبستگی مستقیم و معنی&shy;داری با تغییرات ارتفاع از سطح دریا دارد.</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"></span></span></p> <p dir="RTL"></p> <p><strong>Background & Objective: </strong>Lilium ledebourii (Baker) Boiss, is the only national and natural heritage of Iran, which grows in mountainous regions of Hyrcanian forests with limited distribution that many ecological, ornamental and medicinal aspects of this plant has not been studied yet.<br> <strong>Material & Methods: </strong>Ethanolic extract was prepared by maceration method. Then, total phenol and total flavonoids of ethanolic extracts were respectively performed by the Folin- Ciocalteu and AlCl3 method. Antioxidant activity was measured by scavenging free radicals of DPPH. Statistical analysis of the data was performed by ANOVA (Analysis of Variance) and Duncan Test to compare the means.<br> <strong>Results:</strong> The total phenols of Lily shoots was between 59/6 &plusmn; 65/43 and 21/9 &plusmn; 14/66 and its antioxidant activity was between 51 to 90 percent at four habitats, The highest and lowest amount of total phenols and antioxidant activity of Lily shoots obtained from Kelardasht (maximum altitude) and Rostam Abad (minimum altitude). The total flavonoid were reported between 91/7 &plusmn; 41/33 mg GAE g-1 and 21/1 &plusmn; 72/43 mg QUE g-1. The highest and lowest content of Total flavonoid were respectively observed in Asalem and Namin.<br> <strong>Conclusion:</strong> The results showed that the antioxidant capacity of Lily is relatively high compared to other medicinal plants, also antioxidant activity of the Lily extract is variable in different sites and significantly affected by habitat altitude.</p> سوسن چلچراغ, فعالیت ضد اکسیدانی, فلاونویید, عصاره, رویشگاه Lily, antioxidant activity, flavonoid, extraction, habitat 727 734 http://jabs.fums.ac.ir/browse.php?a_code=A-10-1482-2&slc_lang=fa&sid=1 Shekoofeh Shokrollahi شکوفه شکراللهی shokrollahi.sh93@yahoo.com 100319475328460017657 100319475328460017657 Yes Agriculture Sciences and Natural Resources, Gorgan, Iran دانشکده مرتع و آبخیزداری، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران GholamAli Heshmati غلامعلی حشمتی 100319475328460017658 100319475328460017658 No Agriculture Sciences and Natural Resources, Gorgan, Iran دانشکده مرتع و آبخیزداری، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران Hamed YosefZadeh حامد یوسف زاده 100319475328460017659 100319475328460017659 No Environment, Natural Resources and Marine Sciences, Tarbiat Modares University,Tehran, Iran دانشکده منابع طبیعی و علوم دریایی نور، دانشگاه تربیت مدرس، تهران، ایران