Journal of Advanced Biomedical Sciences
مجله علوم زیست پزشکی پیشرفته
J Adv Biomed Sci.
Medical Sciences
http://jabs.fums.ac.ir
1
admin
2783-1523
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8888
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jalali
1396
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1
gregorian
2017
9
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online
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fulltext
fa
آنالیز مولکولی کلاس های مختلف اینتگرون در سویه های بالینی پسودوموناس آئروژینوزا با استفاده از روش Multiplex-PCR
Molecular analysis of various classes of integrons in the clinical Pseudomonas aeruginosa isolates by Multiplex-PCR
ميكروبیولوژی
Microbiology
پژوهشي
Research
<div><strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">زمینه و هدف: </span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">حضور</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اینتگرونهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلاس </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">I</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">II</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">III </span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></span> <em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پسودوموناس</span></span></span></em> <em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آئروژینوزا</span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مقاومت دارویی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">چندگانه،</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نگرانیهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فراوانی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلینیک</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ایجاد</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کرده</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">است.</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">باهدف</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فراوانی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلاس </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;"> I</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">II</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">III</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ایزولههای <em>پسودوموناس آئروژینوزا </em>انجام</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">.</span></span></span></div>
<p dir="RTL"><br clear="all" >
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مواد و </span></span></span></strong><strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">روشها</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">: در این مطالعه توصیفی </span></span></span><span style="color:black;"><span style="font-family:arial,sans-serif;"><span style="font-size:10.0pt;">-</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> مقطعی، 60 ایزوله <em>پسودوموناس آئروژینوزا</em> از آزمایشگاه میکروبشناسی بیمارستان حضرت رسول (ص)، تهران به دست آمد. تست حساسیت آنتیبیوتیکی به روش انتشار در ژل و بر اساس دستورالعمل </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">CLSI</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و بر روی محیط مولر هینتون آگار برای آنتیبیوتیکی انجام گردید. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">همچنین </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">DNA</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> ژنومیک سویهها با استفاده از کیت استخراج و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">M-PCR</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> با استفاده از آغازگرهای اختصاصی </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">intI</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">، </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">intII</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">intIII</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> انجام گردید.</span></span></span><br>
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتایج</span></span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">:</span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بیشترین میزان مقاومت در سویههای واجد اینتگرون بوده، بهطوریکه تمامی سویهها به آموکسی سیلین (6/96%) مقاوم بودهاند. نتایج نشان داد تمامی 60 سویه تحت مطالعه دارای مقاومت کامل (%100) به آمیکاسین بودند. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بیشترین توزیع فراوانی مربوط به ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">intI</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> با </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">40</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">% و کمترین فراوانی ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">intIII</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> به میزان 6/</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">16</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">% گزارش شد. ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;"> intII</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در هیچیک از نمونه­های مورد بررسی شناسایی نگردید.</span></span></span><br>
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتیجهگیری:</span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">توجه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شیوع</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بالای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کلاس</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;"> I </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ایزولههای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مقاوم</span></span></span> <em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پسودوموناس آئروژینوزا</span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ارتباط</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آن</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">الگوهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مختلف</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مقاومت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دارویی،</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اعمال</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">راهکارهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مناسب</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کنترل عفونت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">درمان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بیمارستانهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مورد</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">برای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">جلوگیری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">انتشار</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بیشتر</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آنها</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ضروری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نظر میرسد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:8.0pt;">.</span></span></span></p>
<p><strong>Background & Objective: </strong>The presence of classes I, II and III Integrons in multidrug-resistant <em>Pseudomonas aeruginosa</em> strains, has created new concerns in clinics. This study aimed to investigate the prevalence of class I, II and III Integron in clinical <em>Pseudomonas</em> <em>aeruginosa</em> isolates.<br>
<strong>Materials & Methods:</strong> In the cross-sectional study, 60 isolates of <em>P. aeruginosa</em> were obtained from the microbial-Laboratory of Rasool Akram Hospital in Tehran. Antibiotic susceptibility test was performed by the agar diffusion method based on CLSI guidelines on the Mueller Hinton agar medium. The genomic DNA was extracted using DNA extraction kit and M-PCR was performed for amplification of <em>intI</em>, <em>intII</em> and <em>intIII</em> genes.<br>
<strong>Results: </strong>The highest resistance rate was in the strains that harbored integron, so that 96.6% of strains were resistant to amoxicillin. The results showed that all 60 strains had resistance (100%) to amikacin. The highest frequency was related to <em>intI</em> (40%) and the lowest frequency was related to <em>intIII</em> genes (16.6%).<br>
<strong>Conclusion: </strong>Considering the high prevalence of class I Integron in all <em>Pseudomonas</em> <em>aeruginosa-resistant</em> isolates and its relation with different drug resistance patterns, the right solution to infection control and treatment in hospitals is essential to prevent further spread of it.<br>
</p>
ژنهای اینتگرون کلاس I, II, III, پسودوموناس آئروژینوزا, .Multiplex-PCR
Class I, II, III Integron genes, Pseudomonas aeruginosa, Multiplex-PCR.
210
216
http://jabs.fums.ac.ir/browse.php?a_code=A-10-839-2&slc_lang=fa&sid=1
Atousa
Rashidi
آتوسا
رشیدی
alm3370@yahoo.com
100319475328460017878
100319475328460017878
No
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران
Gholamali
Moradli
غلامعلی
مرادلی
moradli.mic@gmail.com
100319475328460017879
100319475328460017879
Yes
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران