TY - JOUR JF - JABS JO - JABS VL - 7 IS - 4 PY - 2017 Y1 - 2017/12/01 TI - The Effect of Curcumin on Pituitary-Gonadal Axis, DNA Oxidative Damage and Antioxidant Enzymes Activity of Testicular Tissue in Male Diabetic Rats TT - اثر کورکومین بر محور هیپوفیز-گناد، آسیب اکسیداتیو DNA و فعالیت آنزیم‌های آنتی‌اکسیدانی بافت بیضه در موش‌های صحرایی دیابتی N2 - Background & Objective: Diabetes causes reproductive disorders by endocrine glands dysfunction. Also curcumin has antioxidant and hypoglycemic properties. The purpose of this study was to determine the effect of curcumin on pituitary-gonadal axis, DNA oxidative damage and antioxidant enzymes activity of testicular tissue in male type one diabetic rats. Material & Methods: In this experimental study 40 male Wistar rats were allocated into 5 equal groups (n=8). Control group, non-treated diabetic group (30 days, intraperitoneal injection of dimethyl sulfoxide) and treated diabetic groups with doses 50, 100 and 150 mg/kg of curcumin (30 days, intraperitoneal injection of curcumin dissolved in dimethyl sulfoxide). Diabetes was induced using an intraperitoneal injection of 240 mg/kg alloxan. At the end of treatments, serum levels of LH, FSH, estrogen, testosterone, dihydrotestosterone and levels of SOD, CAT, GPX, HOdG-8 and MDA in testicle tissue were measured by ELISA. The statistical analysis was carried out using one-way ANOVA and post-hoc Tukey tests (p<0.05). Result: Compared to the non-treated diabetic group, administration of curcumin with doses of 100 and 150 mg/kg caused dose-dependent significant increased serum levels of LH, FSH, estrogen, testosterone and dihydrotestosterone, dose-dependent significant decreased HOdG-8 and MDA and also dose-dependent significant increased enzyme of SOD, CAT, and GPX in diabetic rats’ testicle (p<0.05). Conclusion: Administration of curcumin increases pituitary-gonadal axis activity, decreases lipid peroxidation and DNA oxidative damage and also increases antioxidant enzyme activity of testicular tissue in diabetic rats. SP - 511 EP - 520 AU - Sadoughi, Seyed Damoun AU - Edalatmanesh, Mohammad Amin AU - Rahbarian, Raheleh AD - Department of Biology, College of Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran KW - Diabetes Mellitus KW - Curcumin KW - Testis KW - Antioxidant Enzymes KW - Rat UR - http://jabs.fums.ac.ir/article-1-1287-en.html ER -