Search published articles


Showing 8 results for Tumor
Evaluation of Th1 and TH2 Cytokine Network in Aspergilus Infected Tumor Bearing Mice
Noushin Sohrabi, Majid Tebyanyan, Mehdi Mahdavi,
Volume 2, Issue 1 (5-2012)
Abstract

Background & Objective: Invasive Aspergillosis is a fatal infection in immunocompromised patients. The aim of this study was to evaluate the interaction of infectious with Aspergillus and tumor on immune response and cytokine network in Aspergillus infected tumor-bearing mice. Materials and Methods: Mice were implanted by sterile pieces of mouse mammary tumor and then infected by Aspergillus conidia by IV injection. Control groups mice were infected with Aspergillus conidia and/or implanted by sterile funga pieces of mouse mammary tumor. Seven days after Aspergillus infection, cytokine production of extracted splenocytes was analyzed by ELISA method.

Results: Tumor bearing mice which were infected with Aspergillus conidia showed remarkable increase in IL-4 production. Conversely, the levels of IFN-γ and IL-10 were decreased and TNF-α was increased moderately.

Conclution: Probably, Aspergillus infection could change cytokine production from CD4+ T helper cells and acquired immunity of tumor bearing mice. This point may be considered for better management of Aspergillus-infected patients suffering from cancer.


Evaluation of Antitumor Activity of Valproic Acid on K562 cells: In Vitro
Shahin Aalaei Diman, Mehdi Mohamadzade, Yaghub Pazhang,
Volume 6, Issue 3 (11-2016)
Abstract

Backgrond & Objective: This study was performed to evaluate the antitumor effects of valproic acid (VPA) drug on K562 cells in vitro.

Material & Methods: For this purpose, K562 cells were cultured and treated with different doses of valproic acid, and its antitumor properties were measured by MTT assay 24, 48, and 72 hours after treatment. Then, DNA electrophoresis and staining with Hoechst were used to analyze apoptotic cells in vitro.

Results: The antitumor effects of valproic acid increased in a dose- and time- dependent manner. Also, the apoptotic effects of the drug were approved by electrophoresis. The maximum inhibitory effect was observed in higher concentration and after 72 hours treatment. IC50 was calculated 80Mµ by Compusyn software. The results showed that valproic acid was an efficient drug for inhibiting K562 cells, and its antitumoric effect would warrant further.

Conclusion: Accordingly, valproic acid is suitable for inhibiting K562 cells, and these antitumoric properties would warrant further studies on the clinical application of valproic acid. Therefore, this drug might be effective for the prevention and treatment of chronic myeloid leukemia.


New techniques and methods in the study of the invasion, cell migration and MMPs activity in vitro and in animal models
Ali Golestani, Raziyeh Abdollahipour Haghighi, Mohammad Ali Takhshid, Rita Arabsolghar,
Volume 8, Issue 4 (12-2018)
Abstract

Background & Objective: Cancer metastasis is the primary cause of cancer morbidity and mortality, it accounts for about 90% of cancer deaths. Cancer treatment has improved significantly, due to early detection and inhibition of cancer growth.
The ability to invade and migrate is important in malignant tumor cells. The study of cell migration is valuable in cancer diagnosis, prognosis, drug development and treatment. New methods are available to investigate the invasion, migration and the activity of enzymes involved in the invasion process in the laboratory. The effectiveness and efficacy of various anti-cancer drugs and compounds can be studied using these methods in laboratory and animal models.
Conclusion: In this paper, we offer a summary of in-vitro migration assays, including the transwell migration assay, scratch wound assay, microfluidic chamber assay, exclusion zone assay, fence assay, micro carrier bead assay, spheroid migration assay and in-vivo approach, Chick chorioallantoic membrane (CAM) assay.
This review also provides an overview of methods, In situ Zymography, ELISA, and FRET based measurement of MMP activity and Substrate Zymography for measuring the level of metalloproteinase enzyme as the major enzyme in the degradation of extracellular matrix.

 


The role of histone deacetylases in human gastrointestinal and associated glands cancer
Masumeh Sanaei, Fraidoon Kavoosi,
Volume 9, Issue 3 (9-2019)
Abstract
Background & Objective: In the eukaryotic cells, the DNA wraps around the histone proteins (H2A, H2B, H3, and H4) and constitutes the nucleosome. Chromatin organization plays a major role in the control of gene expression. Epigenetic modifications can induce a reversible change in the chromatin structure as being open and accessible DNA to the transcriptional factors resulting in gene expression. Histone modification, as an epigenetic factor, is necessary for gene expression. These modifications are concluded in a balance between histone acetyltransferase and histone deacetylase activity. Histone deacetylation compacts chromatin structure resulting in gene silencing. Tumor suppressor genes play an important role in the cancer prevention. Deacetylation of these genes resulting in genes silencing and carcinogenesis. In this review, we will evaluate the effects of histone deacetylases on the gastroinetestinal tract and associated glands cancer.
Methods: For this review article, we search different online sources by various researcher motors including Scopus, PubMed, and ISI resulted in finding articles correlated with the effect of histone deacetylase on the gastroinetestinal tract and associated glands cancer.
Conclusions: In the current study, we concluded that histone deacetylases can induce cancer by histone deacetylation of tumor suppressor genes in the gastrointestinal tract and associated glands.
 
Angiogenesis and Metastasis with a Therapeutic Approach: A Review
Dariush Rostami, Elahe Abdollahi, Hassan Taghizadeh, Sayed Mohammad Gheibi Hayat, Eskandar Taghizadeh,
Volume 9, Issue 3 (9-2019)
Abstract

Angiogenesis is a process in which new blood vessels produced from old vessels and this process plays an important role in the natural processes in body. Also, angiogenesis in tumor cells leads to further growth of these cells and leads to their metastasis. This process involves migration, proliferation and differentiation of endothelial cells. One of the new ideas that attracted the attention of scientists is to inhibit the angiogenesis process in cancer patients. Scientists are hoping that this method leading to a tumor cells therapy. The purpose of this study was to review the role of angiogenesis in metastasis of tumor cells with a therapeutic approach.


Poly (Lactic Acid) Nanoparticles: A Promising Hope to Overcome the Cancers
Farnaz Fattahi, Tahereh Zamani,
Volume 11, Issue 2 (5-2021)
Abstract
Nanoparticles are used mainly for the transmission of the therapeutic molecules (like drugs, proteins, or DNA) to the organ/tissue of human body. Polymeric nanoparticles are mostly applied for therapeutic effectiveness in cancer therapy. The micro environment of tumor tissues in vessels can assist nanoparticles in achieving their anticipated accumulation. Poly (lactic acid)(PLA) is a novel green polymer with natural sources (like sweet potato and sugar cane). PLA is a linear aliphatic which has great sustainability, renewability and compostability. PLA has popper mechanical, thermal and barrier properties. This biomaterial is thermoplastic polyester with biocompatibility, non-toxicity and biodegradability. Various forms of PLA nanoparticles are synthetized for biomedical applications like cancer treatments and wound healing process. This review article introduces the various structures of polylactic acid nanoparticles used to deliver anticancer drugs. Furthermore, the investigational approaches that are considered for using PLA nanoparticles in treatment of different types of cancers will be reported briefly.
 

Investigating the Relationship between Serum Glucose and Inflammatory Markers in Diabetic Rats with Progressive Resistance Exercise
Maryam Vatandoust, Ali Zare,
Volume 11, Issue 4 (11-2021)
Abstract
Background & Objective: Diabetes Mellitus (DM) is a condition that is associated with an increase in inflammatory markers and studies show that resistance exercise with appropriate intensity can reduce inflammation generally. The aim of this study was to investigate the relationship of serum glucose with Interlekin18 (IL-18) and Tumor Necrosis Factor-alpha (TNF-α) in Diabetic rats with progressive resistance exercise.
Materials & Methods: In this experimental study, 32 Wistar male rats were distributed to sedentary control, trained control, sedentary diabetic, and trained diabetic groups of 8. Diabetes was induced by Streptozotocin (55 mcg/bw-i.v.). The resistance training protocol consisted of elevating upward from a ladder with weight, supporting an overload equivalent to 5% of body weight, during 6 weeks. The data obtained were analyzed by ANOVA and Tukey's posthoc multiple comparison tests, and a significance level of 5% was considered.
Results: According to results, serum concentrations of TNF-α and IL-18 decreased significantly in trained diabetic group compared to sedentary diabetic group after resistance training. While in the amount of TNF-α in the trained control group compared to the sedentary control group, a significant increase was observed, and in the amount of IL-18 in trained control group compared to the sedentary control group, a significant decrease was observed. But regarding the correlation between serum IL-18 concentration and glucose, only in sedentary control group, a significant correlation was observed.
Conclusions: Resistance training was able to reduce TNF-α and IL-18 inflammatory markers in trained diabetic rats and improve metabolic and immune aspects in diabetes mellitus.
 

Bioinformatic Analysis of lncRNA H19 Reveals Its Regulatory Networks and Clinical Relevance in Gastric Cancer
Kianoush Mohammadi, Reza Safaralizadeh, Elham Safarzadeh,
Volume 15, Issue 4 (10-2025)
Abstract
Background & Objectives: Gastric cancer (GC) continues to rank among the leading causes of cancer-related mortality worldwide, primarily because of late-stage diagnosis, marked molecular heterogeneity, and the emergence of therapeutic resistance. The long non-coding RNA (lncRNA) H19 has been recognized as an oncogene in multiple malignancies; however, its precise molecular mechanisms and clinical significance in GC remain incompletely understood.
Materials & Methods: We conducted an integrative bioinformatics analysis of 431 TCGA-STAD samples, integrating somatic mutation, RNA-seq, and clinical datasets. The study examined mutational landscapes, tumor mutational burden (TMB), and distinct mutational signatures. Patients were classified according to H19 expression levels for subsequent differential expression, correlation, pathway enrichment, protein–protein interaction (PPI) network construction, and survival analyses.
Results: The most frequent mutations were identified in TTN (51%), TP53 (46%), MUC16 (31%), ARID1A (27%), and LRP1B (27%). Six distinct mutational signatures were detected, reflecting processes associated with aging, mismatch repair deficiency, POLE-driven hypermutation, and prior chemotherapy exposure. Stratification based on H19 expression revealed 15,179 differentially expressed genes that were significantly enriched in pathways related to extracellular matrix organization, focal adhesion, and cell adhesion. H19 exhibited strong positive correlations with IGF2, TCF15, and miR-675, suggesting a potential competing endogenous RNA (ceRNA) function, and negative correlations with ATP4A and ATP4B, indicating possible disruption of parietal cell activity. The hub genes identified within the PPI network included GAPDH, COL1A1, TGFB1, and SIRT1.
Conclusion: Collectively, these findings suggest that H19 acts as a pivotal regulator in GC by modulating ceRNA networks, promoting extracellular matrix remodeling, and influencing oncogenic signaling cascades. Although its independent prognostic significance has yet to be fully established, this comprehensive systems-level analysis provides valuable insights and lays the groundwork for future experimental and clinical studies exploring H19 as a potential diagnostic biomarker and therapeutic target.

Page 1 from 1     

© 2026 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)