Search published articles


Showing 3 results for Polymerase Chain Reaction
Investigating the relationship between Human Papillomavirus and Herpes Simplex Virus Type 1 and 2 with Endometriosis Lesions
Fatemeh Azizvakili, Gita Saadatnia, Mahnaz Hadizade,
Volume 7, Issue 4 (12-2017)
Abstract

Background & Objective: Endometriosis is one of the most common diseases in women, in which endometrial tissue begins to grow outside the uterine. Many factors are involved in the development of this disorder. Studies have shown that infectious agents due to the inflammation may predispose endometriosis. In this study the presence of human papillomavirus and herpes simplex virus types 1 and 2 were examined in endometriosis lesions.
Material & Methods: This case-control study was performed in Sarem Women's Hospital. 40 paraffin-embedded blocks of endometriosis and 40 normal endometrial tissue blocks from patients without endometriosis were selected as control. After DNA extraction, molecular analysis was performed using polymerase chain reaction for diagnosis of mentioned infections.
Results: The results of this study showed that, in investigation of papilloma infection, the virus DNA was found in one of the tissues of patients group (2.5%) and in 6 (15%) of healthy subjects. HSV infection was detected in 5 samples (12.5%) of the endometriosis tissues and 2 samples (5%) of control group.
Conclusions: Findings of this research indicated that there is no significant association between papillomavirus and herpes simplex virus with endometriosis. In the other words, the presence of these viruses as factors that increase the risk of endometriosis incidence was not confirmed (P = 0.14 and P= 0.38, respectively), however further investigations are needed for the final conclusion.
 
 


Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis
Mohammad Soleimani, Mohammadreza Zolfaghari, Abbas Morovvati,
Volume 10, Issue 3 (8-2020)
Abstract
Background & Objective: Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house PCR Method, to detect putative periodontitis-related bacterial pathogens.
Materials & Methods: The PCR method was launched using specific primers of the five bacteria including Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola. Then, the sensitivity and specificity tests were performed for each bacterium after cloning.
 Results: Basic specific Primer: hbp Aggregatibacter actinomycetemcomitans، fimA Porphyromonas gingivalis، gene 16s rRNA Prevotella intermedia 16s rRNA Tannerella forsythensis gene and 16s rRNA Treponema denticola 161 bp، 162 bp, 282 bp, 280 bp,173 bp and the sensitivity and specificity tests were performed for this gene.
Conclusion: In order to evaluate and diagnose periodontal diseases using PCR technique, these factors can be identified with high specificity and sensitivity.
 

Investigating The Expression Changes of miR-93-3p, NFATc1 and NFATc3 Genes in Peripheral Blood Mononuclear Cells (PBMC) of Breast Cancer Patients
Mansoreh Dastgir, Garshasb Rigi, Samira Ghaedmohammadi, Sedigheh Tahmasebi,
Volume 14, Issue 1 (1-2024)
Abstract
Background & Objectives: Breast cancer is the most lethal malignancy in women. miRNAs function as epigenetic regulators and contribute to the pathogenesis of breast cancer. Nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and 3 (NFATc3), are targeted by microRNA-93 (miR-93). This study aims to evaluate the expression of these genes in peripheral blood mononuclear cells (PBMCs) of healthy women and women with breast cancer.
Materials & Methods: In this cross-sectional study, blood samples were collected from 20 healthy women and 20 women with early-stage breast cancer. After isolating peripheral blood mononuclear cells (PBMCs), RNA extraction and cDNA synthesis were performed. The expression of the desired genes was then examined by Real-Time Polymerase Chain Reaction (RT-PCR). Statistical analysis was conducted. A p-value less than 0.05 was considered statistically significant, and Student's t-test was used to evaluate the relative changes in gene expression.
Results: The results demonstrated that the expression of NFATc1 and NFATc3 genes in peripheral blood mononuclear cells (PBMCs) of breast cancer patients was significantly reduced compared to their expression in healthy individuals. Conversely, the expression of the miR-93-3p gene was significantly lower in healthy women than in breast cancer patients (p < 0.05).
Conclusions: This study investigated the expression of miR-93-3p and its downstream targets, the NFATc1 and NFATc3 genes, for the first time in peripheral blood mononuclear cells. The expression levels were shown to be significantly different in patients with breast cancer compared to healthy women.
Keywords:

Page 1 from 1     

© 2025 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)