Search published articles


Showing 4 results for Pathologic
Investigation of curcumin effects on liver tissue in adult male rats treated with cyclophosphamide
Zahra Khodaparast, Ali Reza Yousofi, Ameneh Khoshvagti,
Volume 4, Issue 3 (11-2014)
Abstract
 

Background & Objective: Cyclophosphamide is an antineoplastic drug that has many clinical uses in cancer treatment, but it has toxic effects due to creation of free radicals. In this study, the effects of curcumin" as an antioxidant drug” on liver tissue was investigated.

 

Materials & methods: 50 wistar adult male rats were selected randomly and were divided in to five groups including control, sham (receiving normal saline and olive oil), cyclophosphamide (5 mg/kg intraperitoneal), cyclophosphamide (5 mg/kg) + curcumin (50 mg/kg), and curcumin (50 mg/kg by gavage). This study continued for 30 days. The liver histopathological changes were investigated after the last administration.

 

Results: Severe apoptosis, hepatocytes necrosis, congestion, focal inflammation, and endotheliosis were revealed in cyclophosphamide receiving group. However, focal inflammation and hepatocyte necrosis were decreased considerably in cyclophosphamide + curcumin group compared to cyclophosphamide group. Congestion and endotheliosis were very small. None of the above mentioned complications was observed in curcumin receiving group.

 

Conclusion: The results of this study show the concurrent consumption of cyclophosphamide and curcumin can reduce the cyclophosphamide free radicals effects on liver tissue.

 

 

 
Evaluation of NKILA lncRNA and NFκB Genes Expression in an Iranian Gastric Cancer Patient Compared with Healthy Tissue and the Relationship between Clinicopathological Features
Negar Soltani, Leila Pishkar, Vahid Chaleshi,
Volume 12, Issue 1 (3-2022)
Abstract
Background & Objective: Long non-coding RNAs play critical roles in the initiation and development of gastric cancer (GC). The aim of this study was to assess the expression of NKILA and NFKB genes and the relationship between their expressions with clinical characteristics in gastric cancer. Also, relative expression of lncRNAsNKILA, compared NFKB in GC tissues was evaluated.
Materials & Methods: This cross-sectional study was performed on 50 gastric formalin-fixed paraffin-embedded tumor tissue samples and 50 normal tissues. The RNA level of lncRNAsNKILA and NFKB genes was assessed using the quantitative real-time polymerase chain reaction. B2M was used as an internal control. The 2 -ΔΔCq method was used to determine the expression fold changes.
Results: A significant association was observed between the levels of NKILA, in gastric tumor tissues compared with normal tissues (Mean = 2.087, p= 0.0484). The relative expression of the NFKB gene revealed no statistically significant difference between the gastric tumor tissues compared with normal tissues (P=0.3728). In addition, clinicopathologic data compared with NKILA and NFKBmRNA expression levels in gastric cancer tissues showed no significant association. Here, we found no significant association between the level of NKILA expression compared with NFKB mRNA level in gastric cancer tissues (R=0.03; P=0.2355).
Conclusion: Our results showed that NKILA had a significant association with GC. Our findings also revealed that NKILA expression was not correlated with NFKB mRNA level in GC tissues.
 


Investigation of Lupus Nephritis Cases with Special Reference to Activity and Chronicity Indices
Khashayar Farshid, Farahnaz Noroozinia, Masoumeh Pourjabali,
Volume 13, Issue 2 (5-2023)
Abstract
Background & Objectives: The International Society of Nephrology/ Renal Pathology Society (ISN/RPS) classification is based upon criteria which differentiate acute and chronic phases of Lupus Nephritis. Activity/Chronicity Index grants us a higher insight on the level of pathological lesions and treatment outcome by examining the reversibility of lesions .The present investigation was done in order to highlight the importance of activity and chronicity indices in the course of Lupus Nephritis.
Materials & Methods: Seventy-three Kidney biopsy samples of Lupus Nephritis patients were examined. The information was recorded in a check-list and was then statistically analyzed.
Results: Lupus Nephritis is importantly age-related, frequency of Lupus Nephritis increases with age until the age of 40 years; 21–40 years being the most frequent among studied patients. A prominent decline was seen after the age of 40. Regardless of age, the occurrence was more frequent in females. Class IV was most frequent in all ages and in both sexes. Both Activity and Chronicity scores were slightly higher in females. Activity Index was higher in ages of 11 to 30 years, whereas Chronicity Index was highest in 41 to 50 years of age. The highest Activity Index was reported in Class IV while the highest Chronicity Index was reported in Class V. The least Activity Index was shown in Class VI while Classes II and I had the lowest Chronicity Index. Endocapillary hypercellularity was the most frequent active lesion and tubular atrophy was the most frequent chronic lesion. It is noteworthy that fibrotic crescents were significantly less common among chronic lesions.
Conclusion: Histopathological findings, clinical and para-clinical data could furnish more information on disease process, treatment, quality of life and mortality rate.


Cigarette Smoke-Induced Transcriptomic Alterations and Angiogenesis in Non-Small Cell Lung Cancer: An Integrative Analysis
Kianoush Mohammadi, Reza Safaralizadeh,
Volume 15, Issue 3 (6-2025)
Abstract

Background & Objectives: Cigarette smoke is a major risk factor for non-small cell lung cancer (NSCLC) and plays a pivotal role in tumor initiation and angiogenesis. This study aimed to elucidate the molecular mechanisms through which cigarette smoke influences angiogenesis in NSCLC by integrating transcriptomic data, with a particular emphasis on the regulatory role of microRNA-1 (miR-1) and its downstream targets.
Materials & Methods: We analyzed the Gene Expression Omnibus (GEO) dataset GSE290190, comprising gene expression profiles from 18 samples with different smoking statuses (9 normal and 9 tumor tissues). Differential expression analysis, Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO) enrichment, and Protein–Protein Interaction (PPI) network analysis were conducted to identify critical genes and signaling pathways. Statistical analyses were employed to determine differentially expressed genes (DEGs) and to assess their biological relevance.
Results: Differential expression analysis identified 2,449 DEGs between normal and tumor tissues, with significant enrichment in angiogenesis, cell cycle regulation, and DNA repair pathways. Key angiogenesis-related genes—VEGFC, FGF2, and ANGPT1—were recognized as direct targets of miR-1. GSEA and GO analyses revealed marked alterations in biological processes such as chromosome segregation, mitotic nuclear division, and extracellular matrix organization. PPI network analysis identified E2F7, PLK1, and TOP2A as hub genes, suggesting their potential roles as key regulators in cell cycle progression and tumorigenesis.
Conclusion: This study highlights the transcriptomic heterogeneity of NSCLC and proposes miR-1 and its downstream targets—VEGFC, FGF2, and ANGPT1—as promising biomarkers and therapeutic targets. However, further validation using larger datasets and functional assays is essential to confirm these findings and facilitate their clinical translation.

 


Page 1 from 1     

© 2026 CC BY-NC 4.0 | Journal of Advanced Biomedical Sciences

Designed & Developed by: Yektaweb

Creative Commons License
This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)