Showing 7 results for Il-1
Yaser Mansoori, Ali Moravej, Sohrab Najafipour, Seyed Amin Kouhpayeh, Mohammad Hasan Meshkibaf, Abbas Abdollahi, Manochehr Rasouli, Ghader Allahverdi,
Volume 2, Issue 2 (8-2012)
Abstract
Background & Objective: Host resistance to Leishmania infection is mediated by cellular immune responses leading to macrophage activation and parasite killing. IL-18 known as interferon-γ inducing factor, stimulate IFN-γ production by T-cells. According to the important role of IL-18 in defense against VL and known effect of IL-18 gene polymorphisms on its production, the aim of this study was to investigate the probable relation between IL-18 gene polymorphisms and susceptibility to VL in Iranian patients.
Materials & Methods: The study groups included 118 pediatric patients suffered from VL and 156 non-relative healthy persons from the same endemic area as the patients. In both study groups IL-18 gene polymorphisms at positions -656 G/T, -137 G/C and +105A/C (codon 35/3) were analyzed by PCR-RFLP (Polymerase Chain Reaction - Restriction Fregment Length Polymorphism).
Results: The result showed that the frequency of T allele at position -656 was significantly higher in the controls compared to that in the patients (P=0.047). But in none of the genotypes of IL-18 there was significant difference between patients and controls. In addition, the distribution of ATG haplotype and AGG/ATG haplo-genotype were significantly higher in the controls compared to that in patients with VL (P=0.043and P=0.044, respectively). Furthermore a strong LDs (P<0.001) were detected between the -607, -137 and codon 35/3 SNPs.
Conclusion: In conclusion, this study showed that the frequency of T allele at position -656 and ATG haplotype and AGG/ATG haplogenotype (positions +105, -656 and -137) were significantly higher in the controls. To the best of our knowledge no study has been conducted on IL-18 gene polymorphisms and VL in other countries, therefore, we were not able to compare our results with other investigations, so it seems that more researches in this field on other populations will be worthy.
Gholam-Reza Ameri, Kianoosh Malekzadeh, Zahra Noor-Mohammadi, Mohammad Shekari, Habibollah Turki, Hanieh Solaimani,
Volume 4, Issue 3 (11-2014)
Abstract
Background & Objective: In human malaria, genotype of cytokines affect immune
system. The impact of genotype -137G/C in IL-18 and +874A/T in IFN-γ on the severity
of malaria and the quality of treatment – as factors to form asymptomatic
persons in endemic regions- has been investigated.
Materials & Methods: 100 patients and 102 healthy persons were evaluated
by Nested-PCR and thick blood film. IL-18(G-137C) and IFN- γ (A+874T) were
determined by Single Specific Primer-Polymerase Chain Reaction and ARMS-PCR
methods, respectively. The results were analyzed statistically.
Results: In this research, it has been observed that allele-T in IFN- γ
gene and allele-C in IL-8 gene have protective effects against severity of
Malaria. In addition, it has clearly been found that development and growth
rate of plasmodium in µl of blood as well as anemia induced
malaria significantly reduced in carriers of these alleles (p<0.0001). This effect has also been observed in the quality
of the treatment in such a way that no parasite remained at the end of second
day of treatment in carriers of alleles IFN- γTT and IL-18CC.
Conclusion: These findings issue the impact of immunogenetypes of patients on
treatment quality and asymptomatic patients and parasite reservoir as effective
factors in endemic malaria. Immunogenetypes of people may play as a remarkable
factor to control malaria in endemic areas.
Ar Kazemi, H Agha Alinejad, R Eslami, P Ehsan, R Baghaei, R Dabaghzadeh, M Ghanbarzadeh,
Volume 5, Issue 3 (11-2015)
Abstract
Background & Objectives: Breast cancer is nowadays one of the most harmful threats to women’s health. However, exercise training plays an adjuvant role in breast cancer (Adjuvant also means preventive. So, no need to repeat preventing.). Therefore, the aim of this study is to investigate the effect of 6-week endurance training on the levels of interleukin-8 in the tumor and Interleukin-17 in the serum of mice suffering from breast cancer.
Materials & Methods: In this study, 20 female Balb/C mice were randomly divided into exercise-tumor (RET) and rest-tumor (RRT) groups. The mice were oriented in the environment, and one million estrogen-dependent breast cancer cells (MC4L2) were injected into the top of the right thigh of each mouse. Subsequently, the RET group performed the endurance exercise 5 days per week for 6 weeks. The tumor volume was measured by a digital caliper each week. Finally, the mice were sacrificed, and the tumor tissue was removed and kept in -70°C. Then, ELISA method was performed and the data were collected.
Results: After 6 weeks of training, a significant decrease was observed in the RTE group in the serum level of IL-17 and IL-8 protein in tumor (P< 0.05). These results were consistent with the tumor growth rate.
Conclusion: The findings of the present study indicate that endurance training can reduce IL-8 and IL-17 proteins in the tumor and serum of mice ill with breast cancer. Therefore, the physical activity is utilized as an important factor in the improvement of adjutant therapy along with other therapeutic methods to treat breast cancer.
Abdolreza Kazemi, Behjat Kalantari Khandani, Afsoon Ashraf Ganjoei,
Volume 6, Issue 2 (8-2016)
Abstract
Background & Objective: The regular exercise training is known as a preventive and adjuvant therapy in inflammatory diseases such as cancer. The aim of this study was to determine the effect of the aerobic training on IL-10 and IL-8 levels and the count of white blood cells (WBC) in women with breast cancer.
Material & Methods: The statistical society of the present study included Kerman women with breast cancer. Forty patients with breast cancer were randomly divided into two groups: exercise (n = 20) and control (n = 20). The exercise group performed the endurance training for 8 weeks with the intensity between 40 to 55 percent of the target heart rate. Twenty four hours before the first session and 48 hours after the last session of the exercise protocol, blood samples were taken from both groups and then IL-10 and IL-8 levels in serum were measured by ELISA via a Boster kit.
Results: The results of the present study showed that 8 weeks of the endurance exercise training did not significantly increase the IL-10 (P=0.113) serum level, but increased the WBC count (p=0.019) and decreased the serum level of IL-8 (p=0.03) significantly.
Conclusion: According to the results of the present study, regular endurance training via decreasing the inflammatory factors can be considered as an effective factor along with other therapies in improving breast cancer.
Sirous Naeimi,
Volume 8, Issue 1 (4-2018)
Abstract
Background & objective: Studies have shown that increased methylation of CpG islands is one of the important mechanisms in gene silencing. Protein P16 / Ink4 plays an important role in the negative regulator of cell cycle process. Inflammation, including factors that affect gene methylation and IL-17, as an inflammatory cytokine, can play a role in this case. This cytokine gene has several polymorphisms which are involved in the expression of it. According to the statement, the purpose of this study is to investigate IL-17 gene polymorphism on gene promoter methylation of P16 / Ink4 and its relation to breast cancer diseases.
Material & Methods: In this case - control study, a total of 40 Women with Breast cancer and 40 healthy women on September 2015 were examined. DNA was extracted and for gene promoter methylation, MSPCR method was used. Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method. Data were compared in both groups by using Pearson’s chi-square and Hardy-Weinberg equilibrium test.
Results: Results confirm the fact that, there is a relationship between P16/Ink4 gene promoter methylation and breast cancer disease So that, the promoter of P16/Ink4 gene in healthy individuals was much more unmethylated than patients (p<0.05). On the other hand there is no significant difference between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05).
Conclusion: It seems that increases of P16/Ink4 gene promoter unmethylation in control subjects is associated with the likelihood of being resistant to breast cancer.
Pooran Adyani, Asieh Abbassi-Daloii, Saeedeh Shadmehri,
Volume 9, Issue 4 (11-2019)
Abstract
Background & Objective: The aim of this study was to investigate the effect of endurance training and L-carnitine consumption on TNF-a and IL-1β gene expression of heart tissue in Wistar male rats following anabolic steroid consumption (Boldenone).
Materials & Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups including control, no-treatment, boldenone (5mg/kg), L-carnitine and aerobic training- L-carnitine. The endurance moderate intensity training program (55-50% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Injection was conducted once a week, on an appointed day, and in the quadriceps and hamstring it was conducted in depth. After anesthesia, autopsy was performed and the heart was isolated. The TNF-α and IL-1β gene expression in the samples was measured by Real Time PCR. Data were analyzed using t-test, One-way ANOVA and post hoc Scheffe at the significant level of P<0.05.
Results: The results showed that there was a significant difference between the mean TNF-α and IL-1β gene expression of heart tissue in male Wistar rats in different groups (P=0.001). The changes in TNF-α and IL-1β gene expression of heart tissue in L-carnitine and Training-L-carnitine groups were significantly lower than those of the no-treatment and boldenon groups (P=0.001).
Conclusion: It seems that supplementation of L-carnitine with regular aerobic training reduces heart tissue damage induced by anabolic androgenic steroids.
Anahita Alipoor Birgani, Nasrin Sartipnia, Seyed Mohammad Mahdi Hamdi, Mohammad Naghzadeh, Javad Arasteh,
Volume 9, Issue 4 (11-2019)
Abstract
Background & Objectives: Infectious diseases caused by bacteria and fungi have affected billions of people worldwide. Throughout human history, infectious diseases have been the leading cause of death and disability. Infectious diseases today account for one-third of all deaths in the world
. The general objective of this study was to investigate the antimicrobial activity of
Scabiosa Olivieri on gram-positive, gram-negative bacteria and fungi, as well as to study its anti-inflammatory properties by investigating the factors of human IL-1 and TNF-α, which ultimately led to the introduction of an antimicrobial agent and new anti-inflammatory drugs with a natural and inexpensive source.
Materials & Methods: First, the extract of the plant was prepared by maceration. Then, the antimicrobial properties of this extract on
E. coli,
Staphylococcus aureus, and
Candida albicans were investigated by MIC. Also, the effect of this extract on the expression of IL-1 and TNFα in human peripheral blood mononuclear cells was evaluated by ELISA.
Results: Scabiosa Olivieri's extract significantly showed anti-inflammatory properties and has antimicrobial activity against Escherichia coli and a mild antimicrobial activity against Staphylococcus aureus. But the antifungal property was not observed.
Conclusion: It seems that
Scabiosa Olivieri's extract can be used as an anti-inflammatory and antimicrobial agent. However, the effects of its use in the in vivo environment and the chemical analysis of its constituent compounds require further research.
