Journal of Advanced Biomedical Sciences

Background & Objective: Skin is of great significance for protecting body against dehydration, bleeding and invading microorganisms. The wounds are prone to bacterial infections and may increase the possibility of scar formation. The aim of this study was to evaluate the effects of phenytoin cream and aqueous Mazu extract on wound healing in rats.

Material & Methods: 30 male Wistar rats weighing 200-180 g were randomly selected and divided into 5 groups. Having created a 2-cm wound in the animal’s neck (epidermal and dermal), the researchers administered normal saline (control), phenytoin cream, aqueous extract of Quercus Infectoria Galls to the groups with concentrations of 5%, 25%, and 50%  to heal them for 21 days. On the 3^rd, 6^th, 9^th, 12^th, 15^th, 18^th, and 2st, day the diameter of the wound was measured. On 21^st day, wound sampling was carried out and prepared for histopathological studies.

Results: Improvement percentage in the group that received 5% aqueous extract was more than other groups and this difference was significant among control groups on the 3^rd, 6^th, 9^th , and 18^th day (P<0.05) and among groups that received phenytoin on the 3^rd and 6^th day (P<0.05. The macroscopic and microscopic results show that the 5% aqueous extract, phenytoin, normal saline, and 25% and 50% extract played the largest role in wound healing respectively.

Conclusion: According to its antioxidant and antimicrobial effects, the 5% aqueous extract of Quercus Infectoria Galls can be important in wound healing and even superior to phenytoin.

Background & Objectives: Today, Infertility problems including lack of follicle formation, among other things, are of paramount importance. The aim of this study was to investigate the effect of hydroalcoholic extract of alfalfa on oxidative stress and ovarian tissue in female rats.
Materials & Methods: In this experimental-interventional study, 24 rats weighing 200-250g were randomly divided into three groups: control (no treatment) and experimental groups (T1 and T2) which received alfalfa extract at 25 and 50mg/kg intraperitoneally for 25 days, respectively. Body weight of animals and their ovarian weight were measured. The left ovary was prepared for histological study. The antioxidant capacity in serum and malondialdehyde in right ovary extracts were also measured.
Results: At the end of the study, the body weight of the animals in both control and experimental groups compared to that of pre-intervention time, but the increase was not statistically significant (P>0.05). The most ovarian weight was observed in T2group (160g) and the lowest weight was in the control group (146g). The primary and secondary follicles and corpus luteum were the highest number in the T2group of alfalfa extract and the lowest number in the control group. The serum antioxidant capacity in T1 and T2groups were about 922 and 937µmol/ml, respectively, and the lowest amount was in the control group with 780µmol/ml and the difference was significant. Malondialdehyde concentration in control and T2gruop were the average of 0.35 and 0.22µmol/ml, respectively (P<0.05).
Conclusion: Hydroalcoholic extract of alfalfa with phytoestrogenic and oxidative stress control effects can enhance ovarian activity in adult rats.