:: Volume 5, Issue 1 (Spring 2015) ::
JABS 2015, 5(1): 102-110 Back to browse issues page
Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats
Zahra Talebanpour bayat1, Heydar Aqababa1, Manzar Banoo Shojaiefard2
1- Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
2- Department of physiology, Fasa University of Medical Sciences, Fasa, Iran. Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran. , shojaeim@sums.ac.ir
Abstract:   (11574 Views)


Background & Objective: Apium graveolens has antioxidant property because of its flavonoid compounds. In the present study, we investigated the hepatoprotective effects of hydroalcoholic extract of Apium graveolens in Sprague Dawley male rats treated with carbon tetrachloride (CCl4).

Materials & Methods: In this study, 48 rats (weighted 180-200 gram) were divided into six groups each having eight rats. The six groups were control, sham, CCl4, and three experimental groups. The experimental groups (4-6) received 1ml/kg CCl4 twice a week and olive oil with the ratio of 1:1 by intraperitoneal injection for 40 days. In addition, they received 2 cc hydroalcoholic extract of Apium graveolens by daily gavage with the concentrations of 200, 400, and 600 mg/kg/cc, respectively. The sham group received only olive oil with the mentioned ratio and the other group received CCl4 and olive oil. At the end, Alkaline Phosphatase (ALP), Aspartate amino Transferase (AST), Alanine amino Transferase (ALT), and the serum concentrations of albumin, total and direct bilirubin were measured and analyzed by using statistical ANOVA Test.


Results: Injection of CCl4 increased the serum levels of liver enzymes and total and direct bilirubin but it decreased the concentration of serum albumin. On the other hand, hydroalcoholic extract of Apium graveolens decreased the liver enzymes and increased albumin level (p<0.001).


Conclusion: The results of this study reveals that the consumption of hydro-alcoholic extract of Apium graveolens maintain the integrity of the liver and protects it against damage.




Keywords: Apium graveolens, CCl4, hepatic protection, rat
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Type of Study: Research | Subject: Physiology
Received: 2015/01/23 | Accepted: 2015/04/18 | Published: 2015/06/20

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Volume 5, Issue 1 (Spring 2015) Back to browse issues page