Journal of Advanced Biomedical Sciences
JABS
Wed, Oct 14, 2026
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Volume 16, Issue 4 (9-2026)
J Adv Biomed Sci. 2026, 16(4): 0-0 |
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Ethics code: IR.IAU.AHAZ.REC.1403.484
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Derakhshan L, Ghasemian S O, Kajbaf F. Comparative Evaluation of Topical Camellia sinensis and Daphne mucronata Versus Silver Sulfadiazine on Cutaneous Wound Healing in Rats: Modulation of TNF-α, Oxidative Stress, and Collagen Remodeling. J Adv Biomed Sci. 2026; 16 (4)
URL: http://jabs.fums.ac.ir/article-1-3239-en.html
URL: http://jabs.fums.ac.ir/article-1-3239-en.html
1- Department of Veterinary Medicine, Sho.C., Islamic Azad University, Shoushtar, Iran
2- Department of Veterinary Medicine, Be.C., Islamic Azad University, Behbahan, Iran ,ommolbanin.ghasemian@iau.ac.ir
2- Department of Veterinary Medicine, Be.C., Islamic Azad University, Behbahan, Iran ,
Abstract: (2 Views)
Background & Objective: Cutaneous wound healing remains a clinical challenge, and herbal extracts may provide therapeutic options for enhancing tissue repair. This study evaluated the topical effects of Camellia sinensis, Daphne mucronata, and their combination on wound healing in rats.
Material & Methods: Forty male Wistar rats with full-thickness excisional skin wounds were randomly allocated to five groups (n = 8): a negative control group, a group treated with 1% silver sulfadiazine, and three groups treated with 5% hydroethanolic ointments containing C. sinensis, D. mucronata, or their combination. Wound healing was assessed on days 3, 7, 14, and 21 using macroscopic and histomorphometric parameters, including wound closure, re-epithelialization, angiogenesis, fibroblast activity, collagen deposition, and inflammatory cell infiltration. On day 21, tissue concentrations of tumor necrosis factor-alpha (TNF-α) and malondialdehyde (MDA), as well as superoxide dismutase (SOD) activity, were measured. Longitudinal outcomes were analyzed using repeated-measures two-way analysis of variance.
Results: All herbal treatments significantly improved wound-healing indices compared with the negative control (P < 0.001). C. sinensis produced the highest wound-healing percentage on day 21 (97.50 ± 0.11%) and reduced MDA and TNF-α concentrations by 60.49% and 58.02%, respectively, while increasing SOD activity by 157.89%. The combined treatment reduced inflammatory cell infiltration and improved collagen organization.
Conclusion: Topical application of C. sinensis, D. mucronata, and their combination improved macroscopic, histomorphometric, and biochemical indicators of wound healing. C. sinensis demonstrated particularly pronounced effects on late-stage wound closure and oxidative and inflammatory responses.
Material & Methods: Forty male Wistar rats with full-thickness excisional skin wounds were randomly allocated to five groups (n = 8): a negative control group, a group treated with 1% silver sulfadiazine, and three groups treated with 5% hydroethanolic ointments containing C. sinensis, D. mucronata, or their combination. Wound healing was assessed on days 3, 7, 14, and 21 using macroscopic and histomorphometric parameters, including wound closure, re-epithelialization, angiogenesis, fibroblast activity, collagen deposition, and inflammatory cell infiltration. On day 21, tissue concentrations of tumor necrosis factor-alpha (TNF-α) and malondialdehyde (MDA), as well as superoxide dismutase (SOD) activity, were measured. Longitudinal outcomes were analyzed using repeated-measures two-way analysis of variance.
Results: All herbal treatments significantly improved wound-healing indices compared with the negative control (P < 0.001). C. sinensis produced the highest wound-healing percentage on day 21 (97.50 ± 0.11%) and reduced MDA and TNF-α concentrations by 60.49% and 58.02%, respectively, while increasing SOD activity by 157.89%. The combined treatment reduced inflammatory cell infiltration and improved collagen organization.
Conclusion: Topical application of C. sinensis, D. mucronata, and their combination improved macroscopic, histomorphometric, and biochemical indicators of wound healing. C. sinensis demonstrated particularly pronounced effects on late-stage wound closure and oxidative and inflammatory responses.
Type of Study: Research |
Subject:
Biochemistry
Received: 2026/08/4 | Revised: 2026/10/14 | Accepted: 2026/09/9 | Published: 2026/09/10
Received: 2026/08/4 | Revised: 2026/10/14 | Accepted: 2026/09/9 | Published: 2026/09/10
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This work is licensed under a Creative Commons — Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)