Journal of Advanced Biomedical Sciences

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Naji S, Karimi E, Homayouni Tabrizi M.  Evaluation of the Antioxidant Gene Expression and Cytotoxicity Potential of Natural Bioactive Compound of Ferutinin Against the Cancer cell Line. JABS 2020; 10 (2) :2252-2260
URL: http://jabs.fums.ac.ir/article-1-1975-en.html

Background & Objective: One of the most common malignancies among Iranian women is breast cancer. One of the strategies in the field of cancer prevention is the activation of an inherent and acquired cancer immune system that has the power to differentiate and select an action. Today, due to the side effects of therapies, numerous attempts have been made to discover natural compounds that have selective power in limiting cancers. The purpose of the present study was to evaluate the anti-tumor function of ferutinin as a ferula-derived plant compound on MDA-MB-231 cell line in breast cancer cells.
Materials & Methods: MTT test was used to study the toxicity of ferutinin against breast cancer cells and compare it with normal cells. Moreover, the morphologic investigation of Ferutinin-treated MDA cells was accomplished with microscopic observations, and the expression of the apoptotic gene was carried out by Real-time PCR.
Results: The results demonstrate that the viability of MDA cells after 24, 48, and 72 hours’ treatment with ferutinin (100 μg / ml) were reported 62.2, 51.8, and 41.73 percent respectively. Furthermore, the gene expression of Catalase increased with the dose-dependent manner in a concentration of 30 μM whereas no cytotoxic effects of ferutinin were observed on normal HDF cell line.

Conclusion: Regarding the lack of cytotoxic effects of ferutinin on normal HDF cells, it is suggested that ferutinin could be an appropriate therapeutic alternative in patients with breast cancer and oxidative stress consequences.