fa ارزیابی اثر ضد باکتریایی نانو ذرات کایتوزان بارگذاری شده با کورکومین نانوتکنولوژی پزشکی Nanomedicine پژوهشي Research <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" dir="rtl" style="margin: 0cm 0cm 10pt text-align: justify"><b><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="line-height: 115% font-family: " times="">نانو سامانه‌های رها کننده دارو ابزارهای قدرتمند درمانی می&shy;باشند. کورکومین ماده موثر زرد چوبه است و ویژگی‌های ضد باکتریایی دارد. این ماده در آب حل نمی‌شود. با استفاده از فناوری نانو می‌توان کورکومین را در آب حل نمود</span><span 10pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" new="" serif="" style="line-height: 115% font-family: " times="">.</span><span 10pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="line-height: 115% font-family: " times="">ماده</span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="line-height: 115% font-family: " times=""> طبیعی و زیست تخریب پذیر است و در ساخت نانو ذرات حمل کننده دارو به کار می‌رود. </span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="line-height: 115% font-family: " times=""><font size="2"><font face="B Nazanin"><span style="mso-spacerun: yes">&nbsp;</span>نانو-داروی حاصل </font></font></span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="line-height: 115% font-family: " times="">به عنوان </span><span 10pt="" b="" class="MsoNormal" dir="rtl" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="" span="" style="font-family: " times=""><font size="2"><font face="B Nazanin"><span style="mso-spacerun: yes">&nbsp;</span>مواد و روش&shy;ها:</font></font></span></b><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times=""> در این </span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">ند.</span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times=""> کورکومین نیز در هنگام ساخت نانو ذرات در آن بارگذاری شد.</span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">به مدت سه روز جهت درمان پوست موش&shy;های </span><span 8pt="" b="" dir="ltr" font-size:="" mso-bidi-font-family:="" new="" serif="" style="font-family: " times="">BALB/c</span><span 10pt="" 8pt="" b="" dir="rtl" font-size:="" lang="FA" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">عفونی شده با <i>استافیلوکوکوس اورئوس</i></span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times="">&nbsp;</span><span .="" 10pt="" b="" class="MsoNormal" dir="rtl" face="B Nazanin" font="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" p="" size="2" span="" style="font-family: " times="">نتایج:</span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">، </span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times="">بار نانو ذرات کایتوزان حاوی کورکومین را 2 </span><span 10pt="" cambria="" font-size:="" lang="AR-EG" mso-bidi-font-family:="" mso-bidi-language:="" serif="" style="font-family: ">&plusmn;</span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times=""> 7 میلی ولت مثبت و اندازه آن&shy;ها را </span><span 10="" 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-font-family:="" mso-hansi-font-family:="" new="" serif="" span="" style="font-family: " times=""> 160 </span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times="">نانومتر تعیین نمود. میکروسکوپ الکترونی گذاره </span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times=""> نیز نشان دادند که</span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times=""> نانو ذرات به شکل کروی می‌باشند. همچنین اندازه‌گیری جذب نوری با اسپکتروفوتومتر </span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">2</span><span 10pt="" b="" dir="ltr" font-size:="" lang="FA" mso-bidi-font-family:="" new="" serif="" style="font-family: " times=""> </span><span 10pt="" font-size:="" lang="FA" mso-bidi-font-family:="" serif="" style="font-family: ">&plusmn;</span><span 10pt="" b="" dir="ltr" font-size:="" lang="FA" mso-bidi-font-family:="" new="" serif="" style="font-family: " times=""> </span><span .="" 10pt="" b="" dir="rtl" face="B Nazanin" font="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" size="2" span="" style="font-family: " times="">باکتری <font size="2"><font face="B Nazanin"><span style="mso-spacerun: yes">&nbsp;</span><i>استافیلوکوکوس اورئوس</i></font></font></span><span 10pt="" b="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times="">&nbsp;</span><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" style="font-family: " times="">می&shy;گردد.</span><b><i><span dir="ltr" font-size:="" lang="AR-EG" new="" serif="" style="font-family: " times="">&nbsp;</span></i></b><b><span 10pt="" b="" class="MsoNormal" dir="rtl" face="Times New Roman" font="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="font-family: " times="">نتیجه&shy;گیری:</span></b><span 10pt="" b="" font-size:="" lang="AR-EG" mso-ascii-font-family:="" mso-bidi-language:="" mso-hansi-font-family:="" new="" span="" style="font-family: " times="">&nbsp;</span><span 10pt="" b="" class="MsoNormal" dir="rtl" face="Times New Roman" font="" font-size:="" lang="FA" mso-ascii-font-family:="" mso-hansi-font-family:="" new="" p="" size="3" span="" style="font-family: " times=""><font size="2">&nbsp;</font></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">Background & Objective:</span></i></b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times=""> Nanomedicine delivery systems are known as potent therapeutictools. In addition to possessing an effective agent of turmeric, Curcumin shows antibacterial properties as well.<span dir="rtl"><font face="Times New Roman" size="2">&nbsp;</font></span>Curcumin is not water-soluble and it can be solved in water via </span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="line-height: 115% font-family: " times="">nanotechnology-base methods</span><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">. Chitosan is a natural and biodegradable substance that is utilized for the Production of Nanoparticles (NPs) carrying drug. In the following investigation, curcumin is loaded in chitosan NPs and ultimately, the resulting nano-drug is studied as an antibacterial agent. <!--stripped--><!--stripped--></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify unicode-bidi: embed direction: ltr"><b><span style=&quot;line-height: 115% font-family: &quot; b=&quot;&quot; nazanin&quot;=&quot;&quot; font-size:=&quot;&quot; 10pt=&quot;&quot; mso-ascii-font-family:=&quot;&quot; &quot;times=&quot;&quot; new=&quot;&quot; roman&quot;=&quot;&quot; mso-hansi-font-family:=&quot;&quot; roman&quot;&quot;=&quot;&quot; dir=&quot;rtl&quot;><span style="mso-spacerun: yes"><font face="B Nazanin" size="2">&nbsp;</font></span></b><b><i><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">Materials & Methods:</span></i></b><b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">In this study, NPs are produced using chitosan and Tripolyphosphate (TPP) salt</span><span 10pt="" font-size:="" mso-fareast-font-family:="" new="" serif="" style="line-height: 115% font-family: " times="">. Curcumin solution was loaded in </span><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">chitosan NPs during their production. Next, the skins of BALB/c mice infected with <i>staphylococcus aureus</i> are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards, in order to evaluate the antibacterial property of the nano-drug, these skin suspensions of mice are cultured in bacterial medium. <!--stripped--><!--stripped--></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">Results: </span></i></b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">Dynamic Light Scattering<span dir="rtl"><font face="Times New Roman" size="2">&nbsp;</font></span>(DLS) reveals the charge of + 7 &plusmn; 2 mV and the size of 160 &plusmn; 10 nm in curcumin-loaded chitosan NPs. Moreover, </span><span font-size:="" serif="" style="line-height: 115% font-family: ">Transmission electron microscopy (TEM)</span><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times=""> and atomic force microscopy (AFM) indicates a spiral shape. Therefore, the evaluation of the optical density by spectrophotometry demonstrates that 75 &plusmn; 2 % of curcumin are loaded in chitosan NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly inhibited <font size="2"><font face="Times New Roman"><em><span style="background: white">staphylococcus aureus</span></em> <em><span style="background: white">growth. </span></em><!--stripped--><!--stripped--></font></font></span></p> <p class="MsoNormal" style="margin: 0cm 0cm 10pt text-align: justify unicode-bidi: embed direction: ltr"><b><i><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">Conclusion:</span></i></b><b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">&nbsp;</span></b><span font-size:="" new="" serif="" style="line-height: 115% font-family: " times="">This study demonstrates that curcumin<span dir="rtl" lang="FA"><font face="Times New Roman" size="2">-</font></span>loaded chitosan NPs can be applied as a potent agent in treatment of bacterial skin<i>&nbsp;</i>infections</span><font face="Calibri"><font size="3">.<!--stripped--><!--stripped--></font></font></p> <p><font face="Times New Roman" size="3">&nbsp;</font><font face="Times New Roman" size="3">&nbsp;</font></p> نانو ذرات, کورکومین, کایتوزان , استافیلوکوکوس اورئوس Nanoparticles, Curcumin, Chitosan, staphylococcus aureus 134 141 http://jabs.fums.ac.ir/browse.php?a_code=A-10-529-1&slc_lang=fa&sid=1 Mirza Ali Mofazzal Jahromi میرزا علی مفضل جهرمی alimofazzal@yahoo.com 100319475328460011505 100319475328460011505 No Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. مرکز تحقیقات بیولوژی مولکولی، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. Hajar Rajayi هاجر رجایی 100319475328460011506 100319475328460011506 No Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. گروه ایمنی‌شناسی پزشکی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس، تهران، ایران. Sharafaldin Al-Musawi سید شرف الدین موسوی 100319475328460011507 100319475328460011507 No Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. گروه نانوبیوتکنولوژی، دانشکده علوم پایه، دانشگاه تربیت مدرس، تهران، ایران. Majid Pirestani مجید پیرستانی 100319475328460011508 100319475328460011508 No Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. گروه انگل‌شناسی پزشکی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس، تهران، ایران. Mahdi Fasihi Ramandi مهدی فصیحی رامندی 100319475328460011509 100319475328460011509 No Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. مرکز تحقیقات بیولوژی مولکولی، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. Kazem Ahmadi کاظم احمدی 100319475328460011510 100319475328460011510 No Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. مرکز تحقیقات بیولوژی مولکولی، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. Vahid Sharifzadeh Peivasti وحید شریف زاده پیوست 100319475328460011511 100319475328460011511 No Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. گروه باکتری‌شناسی پزشکی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس، تهران، ایران. Zuhair Mohammad Hassan زهیر محمد حسن 100319475328460011512 100319475328460011512 No Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. گروه ایمنی‌شناسی پزشکی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس، تهران، ایران. Mahdi Kamali مهدی کمالی 100319475328460011513 100319475328460011513 No Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. مرکز تحقیقات نانوبیوتکنولوژی، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران. Reza Mirnejad رضا میرنژاد rmirnejad@yahoo.com 100319475328460011514 100319475328460011514 Yes Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. مرکز تحقیقات بیولوژی مولکولی، دانشگاه علوم پزشکی بقیه ا...(عج)، تهران، ایران.