en اثرات تیموکینون بر بیان ژن‌های NPY و AgRP در هیپوتالاموس موش‌های صحرایی سالم و مدل کم‌کاری تیروئید فيزيولوژي Physiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Background & Objective:</span></span></i></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Thymoquinone is the most important compound of <i>Nigella sativa</i>, which stimulates the </span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">activity </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">of thyroid axis in healthy individuals and people with hypothyroidism. The present study </span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">investigated </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">the effects of thymoquinone on N<i>europeptide Y (NPY) and Agouti-dependent peptide (AgRP)</i> gene expression in the hypothalamus of healthy individuals and hypothyroidism- model rats </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Material & Methods</span></span></i></b><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">:</span></span></i></b> <span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Twenty m</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ale Wistar rats weighing 190- 220g were used. </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Hypothyroidism</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> was induced by a daily consumption of Methimazole (20mg/kg) for 42 days via drinking water. Control rats received the intraperitoneal injections of saline</span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">. Intact or </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">hypothyroid rats received the intraperitoneal injections of thymoquinone (10mg/kg) for 15 days. </span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">One day after last injection, the thyroid gland and hypothalamic samples were dissected. Thyroid gland samples were used for histological study. R</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">elative gene expression of hypothalamic <i>NPY</i> and <i>AgRP</i> was determined by real-time polymerase chain reaction.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></i></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Thymoquinone significantly declined the <i>NPY</i> and <i>AgRP</i> gene expressions in</span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> the hypothalamus of </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">intact rats in comparison with control group. Induction of hypothyroidism results in a remarkable increase in the <i>NPY</i> and <i>AgRP</i> gene expressions compared to control rats. In hypothyroid rats receiving thymoquinone, the mean relative <i>NPY</i> and <i>AgRP</i> gene expressions showed an insignificant decrease compared to hypothyroid group. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></i></b><b> </b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Because AgRP/NPY signaling pathway exerts inhibitory effects on thyroid gland function, thymoquinone may stimulate thyroid axis activity partly via inhibiting the hypothalamic <i>AgRP/NPY</i> gene expression in intact rats. </span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">In hypothyroidism, used dose of </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">thymoquinone may</span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> not able to cause a </span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">significant decrease in<i> AgRP/NPY</i> gene expression </span></span><span lang="EN-GB" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">due to its increased levels.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br> &nbsp;</div> Thymoquinone, Black Seed, Hypothyroidism, AgRP, NPY, Methimazole 311 318 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2445-3&slc_lang=en&sid=1 Nasrin Ghahremani نسرین قهرمانی nasrin.ghahremani71@gmail.com 100319475328460026555 100319475328460026555 No Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran گروه زیست شناسی، دانشکده علوم، دانشگاه محقق اردبیلی، اردبیل، ایران Fariba Mahmoudi فریبا محمودی f.mahmoudi@uma.ac.ir 100319475328460026556 100319475328460026556 Yes Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran دانشکده علوم، دانشگاه محقق اردبیلی، اردبیل، ایران