en ارزیابی فعالیت بازدارندگی آنزیم آلفا گلوکوزیداز و آنتی اکسیدان عصاره ها ی گیاه کاسه گل سفید گیاهان دارویی Pharmaceutical Plants پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> &alpha;-Glucosidase is one of the main enzymes in the intestinal absorption of carbohydrates. Inhibition of this enzyme can improve postprandial hyperglycemia in diabetic patients. Also, antioxidants can ameliorate diabetes complications resulted from oxidative stress. In this study &alpha;-glucosidase inhibition, antioxidant activity and total phenol content of different extracts and obtained fractions of <em>Moluccella aucheri </em>have been evaluated.<br> <strong><em>Materials & Methods:</em></strong> The ethanol extract of aerial parts of <em>M. aucheri</em> was fractionated using liquid extraction method with petroleum ether, dichloromethane and ethyl acetate, respectively. The extracts and the resulting fractions of <em>M. aucheri</em> were tested against &alpha;-glucosidase enzyme from yeast using an in vitro colorimetric model at &lambda; 405 nm. Antioxidant activity was assessed using two different methods, ferric reducing antioxidant power (FRAP) and the scavenging activity of DPPH radicals&rsquo; methods.<br> <strong><em>Results:</em></strong> The screening results indicated that the antioxidant activity and total phenolic content of the methanol extract were potentially higher than ethanol extract, while the ethanol extract inhibited &alpha;-glucosidase enzyme to a greater extent. Ethyl acetate fraction illustrated potentially the highest antioxidant activity and phenolic content, but its inhibition activity against &alpha;-glucosidase was placed after the petroleum ether fraction. Two previously isolated methoxy flavones; genkwanin, 5-hydroxyl-7, 4&#39;-dimethoxyflavone inhibited the &alpha;-glucosidase enzyme strongly.<br> <strong><em>Conclusion:</em></strong> Since the ethyl acetate extract has both considerable antioxidant activity and &alpha;-glucosidase inhibitory activity, it may be considered as a potential crude drug for diabetes.<br> &nbsp;</div> diabetes, Moluccella aucheri, α-glucosidase, antioxidant 3839 3847 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2840-1&slc_lang=en&sid=1 Mina Doorandishan مینا دوراندیشان mdurandishan@gmail.com 100319475328460026354 100319475328460026354 No Department of Chemistry, Faculty of Basic Sciences, Golestan University, Gorgan, Iran گروه شیمی، دانشکده علوم پایه، دانشگاه گلستان، گرگان، ایران Morteza Gholami مرتضی غلامی mo.gholami@gu.ac.ir 100319475328460026355 100319475328460026355 No Department of Chemistry, Faculty of Basic Sciences, Golestan University, Gorgan, Iran گروه شیمی، دانشکده علوم پایه، دانشگاه گلستان، گرگان، ایران Hossein Mirkhani حسین میرخانی mirkhanh@sums.ac.ir 100319475328460026356 100319475328460026356 No Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات شیمی دارویی و گیاهی، دانشگاه علوم پزشکی شیراز، شیراز، ایران Pouneh Ebrahimi پونه ابراهیمی epouneh@yahoo.com 100319475328460026357 100319475328460026357 No Department of Chemistry, Faculty of Basic Sciences, Golestan University, Gorgan, Iran گروه شیمی، دانشکده علوم پایه، دانشگاه گلستان، گرگان، ایران Amir Reza Jassbi امیررضا جاسبی jassbiar@sums.ac.ir 100319475328460026358 100319475328460026358 Yes Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran مرکز تحقیقات شیمی دارویی و گیاهی، دانشگاه علوم پزشکی شیراز، شیراز، ایران