fa تأثیربخشی شناور فاقد سلول لاکتوباسیلوس کازئی بر بیان ژن اینتگرون کلاس یک در جدایه­های بالینی اسنیتوباکتر بومانی به روش Real time PCR بیوتکنولوژی پزشکی Medical Biotechnology پژوهشي Research <div style="text-align: justify;"><strong><em>Background & Objective:</em></strong> The most common way for the spread of resistance genes and the emergence of multidrug resistance (MDR)-<em>Acinetobacter baumannii</em> species is the presence of genetic elements called integrons. Therefore, in recent years, the use of probiotics has been recommended to reduce the complications of resistant bacteria and treatment failure. Therefore, the aim of this study was to investigate the effect of probiotic <em>Lactobacillus casei</em> supernatant on the expression of class 1 integron gene in <em>A. baumannii</em> isolates by Real time PCR.<br> <strong><em>Material & methods:</em></strong> Sixty clinical samples of blood, respiratory secretions, urine, and ulcers were collected from hospitals in Saveh, Iran. The presence of the <em>intI</em> gene was evaluated by PCR. After treatment with the sub-MIC strains of <em>L. casei</em> supernatant, mRNA was extracted and Real-time PCR was performed to determine the level of integron gene expression and finally the expression level was determined using &Delta;ct method. Expression analysis was performed by relative mRNA expression compared to the standard strain and it was performed in SPSS version 18 using t-test statistical analysis.<br> <strong><em>Results:</em></strong> Out of 12 isolates of <em>A. baumannii</em>, the prevalence of <em>IntI</em> gene was 100%. The fold change for the intI gene was -1.18, indicating that it decreased in the treated group compared to the untreated group (P <0.05).<br> <strong><em>Conclusion:</em></strong> The results showed that the probiotic supernatant was able to reduce the expression of the <em>intI</em> gene; therefore, the use of probiotics as a supplement or alternative therapy in the drug-resistance microbial infections is recommended.<br> &nbsp;</div> Acinetobacter baumannii, Integron, Lactobacillus casei, Real-time PCR 2768 2774 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2583-1&slc_lang=fa&sid=1 Amir Jalilian امیر جلیلیان ventalex88@gmail.com 100319475328460023979 100319475328460023979 No Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران Gholamali Moradli غلامعلی مرادلی moradli.mic@gmail.com 100319475328460023980 100319475328460023980 Yes Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران Kumarss Amini کیومرث امینی Kumarss Amini 100319475328460023981 100319475328460023981 No Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران