en تاثیر عصاره ی هیدروالکلی آویشن شیرازی بر فعالیت مکانیکی کولون ایزوله موش صحرایی نر فيزيولوژي Physiology پژوهشي Research <div style="text-align: justify;"><strong><em><span style="border:none windowtext 1.0pt;padding:0in;background:#FAFAFA;"><span style="color:black;"><span style="font-size:10.0pt;">Background&nbsp;<span dir="RTL">&</span>&nbsp;Objective:</span></span></span></em></strong>&nbsp;<span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Thyme have been used as a medicinal plant traditionally for the treatment of digestive system disorders. In present study, the effect of hydroalcoholic extract of thyme on motility of isolated colon of male rat and its interaction with cholinergic, adrenergic and nitic oxide systems was performed.</span></span><br> &nbsp;<strong><em><span style="border:none windowtext 1.0pt;padding:0in;background:#FAFAFA;"><span style="color:black;"><span style="font-size:10.0pt;">Materials&nbsp;<span dir="RTL">&</span>&nbsp;Methods</span></span></span></em></strong><em><span style="background:#FAFAFA;"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">:</span></span></span></span></em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;"> Ten adult male rats (180- 250 g) were kept in standard condition for one week. Then rats were anaesthetized by ethyl ether and their colon was isolated and divided in to 1 cm strips. The strips were held to a force transducer and inserted to organ bath contained Tyrode solution. The mechanical activity of strips was recorded by Power Lab system with administration of thyme extract (</span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">0.0175 mg/ml</span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">) and its solvent in experimental and sham groups, and after administration of acetylcholine, epinephrine and L-NAME drugs. Data were analyzed by SPSS software and independent-samples T-test.</span></span><br> &nbsp;<strong><em><span style="border:none windowtext 1.0pt;padding:0in;background:#FAFAFA;"><span style="color:black;"><span style="font-size:10.0pt;">Results</span></span></span></em></strong><em><span style="background:#FAFAFA;"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">:</span></span></span></span></em>&nbsp;<span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">The result showed a significant decrease in the mechanical activity of isolated colon after administration of the effective dose of thyme extract in base line and after of administration of acetylcholine.</span></span><br> <span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">There was no significant difference of mechanical activity of the strips at the presence of extract and its solvent after epinephrine and L-NAME administration. </span></span><br> <strong><em><span style="border:none windowtext 1.0pt;padding:0in;background:#FAFAFA;"><span style="color:black;"><span style="font-size:10.0pt;">Conclusion:</span></span></span></em></strong>&nbsp;<span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">It can be concluded that thyme extract caused colon relaxation via inhibition of cholinergic pathway and independent from adrenergic and nitric oxide systems.</span></span></div> Zataria multiflora Boiss, Isolated colon, Mechanical Activity 3735 3740 http://jabs.fums.ac.ir/browse.php?a_code=A-10-617-4&slc_lang=en&sid=1 Aminollah Bahaoddini امین اله بهاالدینی bahaoddinisusc.ac@yahoo.com 100319475328460025384 100319475328460025384 No Department of Biology, Shiraz University, Shiraz, Iran گروه زیست شناسی، دانشگاه شیراز، شیراز، ایران Fereshteh ِِDadfar فرشته دادفر fereshtehdadfar2003@yahoo.com 100319475328460025385 100319475328460025385 Yes Department of Biology, Payame Noor University, Tehran, Iran گروه زیست شناسی، دانشگاه پیام نور،تهران،ایران Mohhamad Ahmadipour محمد احمدی پور Ahmadipoor_m@yahoo.com 100319475328460025386 100319475328460025386 No Department of Biology, Shiraz University, Shiraz, Iran گروه زیست شناسی، دانشگاه شیراز، شیراز، ایران