fa اثرات مایع رویی حاصل از کشت جداگانه و هم زمان سلول‌های فیبروبلاست و ماکروفاژ بر روی اختلالات پارامترهای اسپرم القا شده به وسیله ی پیچش- واپیچش بیضه در رت های نر بالغ بافت شناسي Histology پژوهشي Research &nbsp;<strong>Background & Objective:</strong> Ischemia and Reperfusion (I/R) lead to the production of excessive reactive oxygen species (ROS). In the present study the protective effects of the cell culture conditioned medium (CM) on sperm quality disorder induced by torsion-detorsion (T-D) in adult rat&rsquo;s testis was evaluated.<br> <strong>Materials & methods:</strong> In this study 42 adult rats were divided into seven groups. 1- Normal control group treated without surgery. 2- Laparotomy group, surgery operated without T-D. 3- T-D group, surgery operated along with T-D. 4- T-D+DMEM group, after T-D Dulbecco&#39;s Modified Eagle&#39;s Medium (DMEM) has been injected. 5-7 groups included T-D+F, T-D+M, and T-D+FM, along with T-D injected conditioned medium of fibroblast, macrophage, and fibroblast-macrophage co-culture respectively. All injections were made at a rate of 10&mu;L once after T-D in the rete testis. After 35 days, sperm quality parameters were evaluated and obtained data were compared between groups by one-way ANOVA statistic method and Tukey test post hoc in P<0.05 level.<br> <strong>Results:</strong> The experimental groups that received CM of the fibroblast and macrophage compared to groups T-D and T-D+DMEM there were showed ameliorative effects in comparison to T-D and T-D+DMEM groups. There was not observed any ameliorative effects in the T-D+FM group.<br> <strong>Conclusion:</strong> The results of this study showed the high ability of supernatant of the separate fibroblast and macrophage culture and in improving sperm parameters after testicular T-D.<br> &nbsp; Testicular torsion-detorsion, Fibroblast, Macrophage, Sperm quality, Rat 1564 1576 http://jabs.fums.ac.ir/browse.php?a_code=A-10-2043-1&slc_lang=fa&sid=1 Mansooreh Mirzaei منصوره میرزایی گله کلایی manssrehmirzaei@yahoo.com 100319475328460029398 100319475328460029398 No Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. Rasoul Shahrooz رسول شهروز r.shahrooze@urmia.ac.ir 100319475328460029399 100319475328460029399 Yes Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. Ali Shalizar Jalali علی شالیزار جلالی ali_shalizar@yahoo.com 100319475328460029400 100319475328460029400 No Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. Farshid Sarrafzadeh-Rezaei فرشید صراف زاده رضایی fsrezaei@gmail.com 100319475328460029401 100319475328460029401 No Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. Nariman Mosaffa نریمان مصفا yasamaryan@gmail.com 100319475328460029402 100319475328460029402 No Department of Immunology, Shahid Beheshti University, Tehran, Iran گروه ایمنی شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران