[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Submit :: Contact Us ::
:: Volume 4, Issue 1 (Spring 2014) ::
JABS 2014, 4(1): 67-73 Back to browse issues page
Probable Limitations of Osteoblasts Isolation from the Periosteum and Alveolar Bone in a Dog Model
Sourena Vahabi 1, Rasoul Mofeed, Leila Jabbareh, Maryam Aghalou
1- , ivsure1@gmail.com
Abstract:   (21102 Views)

Background & Objective: Bone defects in jaws create major problems for oral and maxillofacial surgery. To overcome the limitations of Autografts tissue engineering uses autogenus cells and synthetic scaffolds. Type of cells or cell sources have an important effect on the construction which is produced. The aim of this study was to evaluate the feasibility and probable limitations of osteoblast isolation from the periosteum and alveolar bone in an animal model and to compare their probable results quantitatively.

Materials & Methods: Bone and periosteal samples were harvested from interdental septum and retromolar area of 4 dogs. Because no cell was grown new samples were harvested from edentulous ridge and buccal mandibular periosteum. Since no cell was isolated in next step samples were harvested from buccal area of maxillary premolars and explants in cell culture medium. After 2 weeks adherent cells reaching 80% confluent cells were counted and passaged in cell culture flasks.

Results: Despite first good proliferation of periosteal cells of all dogs in P0, their growth was stopped and they become senescence after one week.

Conclusion: The key problems in culture techniques are cell senescence and de-differentiation leading to lose the ability of proliferation. It seems there are probable reasons for isolating of osteoblasts including thermal damage of cells, contamination of culture with fibroblasts, inconsistency of medium and cell requirements, enzymatic damage from enzymes used for cell passage and high donor ages.

Keywords: Osteoblast, periosteum, alveolar bone, cell culturing.
Full-Text [PDF 765 kb]   (2129 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2014/06/11 | Accepted: 2014/06/11 | Published: 2014/06/11
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Vahabi S, Mofeed R, Jabbareh L, Aghalou M. Probable Limitations of Osteoblasts Isolation from the Periosteum and Alveolar Bone in a Dog Model. JABS. 2014; 4 (1) :67-73
URL: http://jabs.fums.ac.ir/article-1-582-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 1 (Spring 2014) Back to browse issues page
Journal of Fasa University of Medical Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4331